中草药2026，Vol.57Issue(9)：3530-3537,8.DOI:10.7501/j.issn.0253-2670.2026.09.021
矩唇石斛居群遗传多样性分析及DNA指纹图谱构建
Genetic diversity analysis and DNA fingerprint construction of Dendrobium linawianum populations
摘要
Abstract
Objective This study employed iPBS molecular marker technology to analyze the genetic diversity and genetic differentiation relationships among 32 Dendrobium linawianum germplasm resources from different regions.Methods A"0,1"matrix was constructed using Excel 2016,and the genetic similarity coefficient(Gs)was calculated using NTSYS-pc 2.10e to construct a UPGMA cluster dendrogram.Additionally,various genetic diversity indices were calculated using Popgene 1.32.Results A total of 84 clear bands were amplified from the six selected iPBS primers,including 76 polymorphic bands,with a polymorphism rate of 90.48%and an average polymorphism information content(PIC)of 0.46,indicating high polymorphism of the iPBS primers.The genetic similarity coefficients ranged from 0.547 6 to 0.965 5,with an average of 0.766 8.At a genetic similarity coefficient of 0.732,the 32 germplasm samples were classified into four populations.The average observed allele number(Na),average effective allele number(Ne),Nei's genetic diversity index(h),and Shannon diversity information index(I)were 1.631 0,1.381 6,0.219 0,and 0.326 7,respectively.Genetic structure analysis revealed that the genetic diversity(Ht),gene differentiation coefficients(Hs)among populations,and genetic differentiation coefficients(Gst)were 0.281 3,0.219 0,and 0.221 3,respectively.Genetic variation among populations accounted for 22.13%of total variation,while genetic variation within populations accounted for 77.87%,indicating that genetic variation was primarily derived from within populations.Furthermore,DNA fingerprinting of 32 germplasm resources was successfully constructed based on 19 polymorphic loci with six primers,which provided a reference for the classification and identification of D.linawianum.Conclusion The genetic diversity of D.linawianum was significantly lower between populations than within populations,and the genetic variation was mainly within populations.The genetic differentiation characteristics of germplasm resources are significantly correlated with their geographical distribution patterns.This study not only elucidates the genetic relationships among different germplasm of D.linawianum and the characteristics of population genetic diversity,but also provides an important theoretical basis for the subsequent scientific conservation and sustainable utilization of D.linawianum germplasm resources.关键词
矩唇石斛/种质资源/iPBS 标记/亲缘关系/遗传多样性Key words
Dendrobium linawianum Rchb.f./germplasm resources/iPBS markers/phylogenetic relationships/genetic diversity分类
医药卫生引用本文复制引用
殷文忆,黄昌艳,陆顺教,邓杰玲,黄玉红,李还,崔学强,张自斌..矩唇石斛居群遗传多样性分析及DNA指纹图谱构建[J].中草药,2026,57(9):3530-3537,8.基金项目
广西重点研发计划项目(桂科AB21220056) （桂科AB21220056）
广西自然科学基金项目(2020GXNSFBA297021) （2020GXNSFBA297021）
设施与休闲农业产业科技先锋队(桂农科盟202512) （桂农科盟202512）
广西特色作物试验站建设(TS202218) （TS202218）
