中国病毒学2026，Vol.41Issue(1)：58-69,12.DOI:10.1016/j.virs.2026.02.001
Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infections
Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infections
Peilan Wei 1Bin Qu 2Yuanyuan Zhang 3Lei Chen 3Zhiwei Xu 3Zhao Chen 3Qier Zhong 4Xindan Xing 3Xinxin Li 3Jingjing Gao 3Yifang He 3Lu Zhang 5Guifei Xie 3Juan Shang 6Xiaoke Guo 6Jiaxin Jiang 3Yongxia Shi 7Jingxian Zhao 8Yanqun Wang 9Jincun Zhao 10Yingkang Jin 11Qingtao Hu 12Airu Zhu 12Zhen Zhuang 12Zhaoyong Zhang 12Shengnan Zhang 3Jiantao Chen 3Xinyi Xiong2
作者信息
- 1. Pediatric Pulmonary Department,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangzhou 510623,China||State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,China
- 2. Shanghai Institute for Advanced Immunochemical Studies,School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China
- 3. State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China
- 4. State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,China
- 5. Health and Quarantine Laboratory,State Key Laboratory of Respiratory Disease of Guangzhou Customs District Technology Center,Guangzhou 510623,China
- 6. GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,China
- 7. Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,China
- 8. State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,China
- 9. State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,China||School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou 511436,China
- 10. State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,China||Shanghai Institute for Advanced Immunochemical Studies,School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China
- 11. Pediatric Pulmonary Department,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangzhou 510623,China
- 12. State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China
- 折叠
摘要
关键词
Pediatric ARTIs/Microbial landscapes/Bronchoalveolar lavage/Metatranscriptomics/Antimicrobial resistanceKey words
Pediatric ARTIs/Microbial landscapes/Bronchoalveolar lavage/Metatranscriptomics/Antimicrobial resistance引用本文复制引用
Peilan Wei,Bin Qu,Yuanyuan Zhang,Lei Chen,Zhiwei Xu,Zhao Chen,Qier Zhong,Xindan Xing,Xinxin Li,Jingjing Gao,Yifang He,Lu Zhang,Guifei Xie,Juan Shang,Xiaoke Guo,Jiaxin Jiang,Yongxia Shi,Jingxian Zhao,Yanqun Wang,Jincun Zhao,Yingkang Jin,Qingtao Hu,Airu Zhu,Zhen Zhuang,Zhaoyong Zhang,Shengnan Zhang,Jiantao Chen,Xinyi Xiong..Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infections[J].中国病毒学,2026,41(1):58-69,12.基金项目
This project was supported by grants from the National Key Research and Development Program of China(2024YFC2310205 to L.Z.),the National Natural Science Foundation of China(82025001,82495200 and 82495203 to J.C.Z.,92369113 and 82172240 to Y.Q.W.),the Guangdong Medical Technology Research Foundation(B2022233 to Y.K.J.),the Guangzhou National Laboratory and State Key Laboratory of Respiratory Disease(GZNL2024B01001 to Y.Q.W.,and GZNL2023A01004 to Y.K.J.),the Guangdong Basic and Applied Research Projects(2023B1515020040 to Y.Q.W.),the State Key Laboratory of Respiratory Disease(SKLRD-Z-202411 to L.Z.,SKLRD-OP-202309 to Y.Q.W),the Science and Technology Planning Project of Guangzhou City(2024A03J1230 to J.C.Z.,2025B04J0006 to L.Z.),and the Science and Technology Project of General Administration of Customs,P.R.China(2025HK221,2023HK065 to L.Z.).We thank the Biobank for Respira-tory Disease in the National Clinical Research Center for Respiratory Disease(BRD-NCRCRD,Guangzhou,China). （2024YFC2310205 to L.Z.）
