中国病理生理杂志2026，Vol.42Issue(4)：737-748,12.DOI:10.3969/j.issn.1000-4718.2026.04.012
LINC00885促进宫颈癌细胞迁移并上调HPV E6癌蛋白
LINC00885 facilitates cell migration and upregulates HPV E6 oncopro-tein in cervical cancer
摘要
Abstract
AIM:To assess the role and mechanism of LINC00885 in human papillomavirus(HPV)-positive cervical cancer(CC).METHODS:LINC00885 expression profile and survival curve were analyzed in gene expression profiling interactive analysis(GEPIA)database in 306 cervical squamous cell carcinoma(CESC)tissues and 13 normal cervical tissues.The clinical pathological data and LINC00885 expression levels in CESC were downloaded from The Can-cer Genome Atlas(TCGA)for analysis.RT-qPCR was used to analyze differences in LINC00885 expression between nine pairs of CC and adjacent non-cancerous tissues and between HPV-positive CC cell lines HeLa(HPV18+)and CaSki(HPV16+)and HPV-negative CC cell line C33A.The pcDNA3.1/LINC00885 plasmid was constructed and transfected in-to HeLa and CaSki cells,establishing LINC00885 overexpression,empty vector(pcDNA3.1),and blank control groups.We analyzed the effects of LINC00885 overexpression on CC cell proliferation and migration.Sequence manipulation suite open reading frame(ORF)finder was used to predict the ORF of LINC00885.pcDNA3.1-Wt_ORF-HA(wild type),pcD-NA3.1-Mut_ORF-HA(ATG→ATT mutation),and pcDNA3.1-LINC00885 full-length vectors were constructed.These vectors were transfected into HeLa and CaSki cells for overexpression,with an empty vector control established.RNA abundance was compared by RT-qPCR,cell viability was assessed by CCK8,and hemagglutinin-ORF fusion protein ex-pression was confirmed by Western blot and immunofluorescence.LINC00885 was transiently overexpressed in CC cells,with an empty vector control established.RT-qPCR and Western blot were used to assess HPV gene expression in HPV-positive cells(HeLa and CaSki).HeLa cells transiently overexpressing LINC00885 were collected for transcriptome se-quencing to analyze the differentially expressed genes and associated signaling pathways.Expression alterations of the dif-ferential gene U2 small nuclear RNA auxiliary factor 1(U2AF1)were verified using RT-qPCR and Western blot.RE-SULTS:LINC00885 was significantly upregulated in CC tissues and HPV-positive CC cells(P<0.05,P<0.01,respec-tively).LINC00885 upregulation significantly enhanced the migration ability of CC cells(P<0.01).Although LINC00885 contains an ORF,it does not encode short peptides.LINC00885 overexpression significantly upregulated HPV16/18 E6 in HeLa and CaSki cells(P<0.01 and P<0.05,respectively)and increased U2AF1 mRNA(P<0.05)and protein levels.CONCLUSION:LINC00885 upregulates HPV E6 gene and protein expression through non-coding regula-tory pathways,potentially facilitating cancer initiation,progression,and migration,and may serve as a potential biomark-er or therapeutic target.关键词
长链非编码RNA/LINC00885/人乳头瘤病毒/宫颈癌Key words
long non-coding RNA/human papilloma virus/LINC00885/HPV E6 gene/cervical cancer分类
医药卫生引用本文复制引用
李绍颖,薛向阳,Sitraka MANJAKA,朱珊丽,项丹,沈淑蓉..LINC00885促进宫颈癌细胞迁移并上调HPV E6癌蛋白[J].中国病理生理杂志,2026,42(4):737-748,12.基金项目
温州市科技局资助项目(No.Y2023072) （No.Y2023072）
