中国草地学报2026，Vol.48Issue(4)：15-29,15.DOI:10.16742/j.zgcdxb.20250306
湖南稷子种质资源苗期耐盐性鉴定及耐盐种质筛选
Identification of Salt Tolerance and Screening of Salt-Tolerant Germplasm in Echinochloa frumentacea at Seedling Stage
摘要
Abstract
This research evaluated 18 accessions of Echinochloa frumentacea(Roxb.)Link germplasm in a pot experiment under 150 mmol/L NaCl stress at the seedling stage to screen for salt tolerance and establish a corresponding evaluation system.Twenty-four phenotypic traits,encompassing biomass,anti-oxidant enzyme activity,chlorophyll fluorescence parameters and photosynthetic indices,were measured.The comprehensive salt tolerance index(D-value)of each accession was subsequently calculated.Principal component analysis(PCA),correlation analysis,membership function analysis,cluster analysis,and a comprehensive evaluation were then undertaken to systematically assess and classify the salt tolerance of the accessions.A predictive regression model for salt tolerance was developed using stepwise regression analysis.The results revealed significant differences between the salt-treated and control groups for all traits,with varying degrees of correlation among the 24 indicators.Based on the D-value-based hierarchi-cal cluster analysis,the 18 accessions were classified into four groups:two highly salt-tolerant accessions,ten salt-tolerant accessions,five moderately salt-tolerant accessions,and one salt-sensitive accession.Among the highly salt-tolerant group,accession CF033297 exhibited superior salt tolerance and was identified as an elite germplasm resources for breeding salt-tolerant Echinochloa frumentacea Stepwise regression analysis further iden-tified qP,K+,Pn,Fv/Fm,Proline content,O-2,aboveground biomass,and root length as reliable indicators for evaluating seedling-stage salt tolerance in Echinochloa frumentacea.关键词
湖南稷子/种质资源/苗期/耐盐性/综合评价Key words
Echinochloa frumentacea/Germplasm resources/Seeding stage/Salt tolerance/Compre-hensive evaluation分类
农业科技引用本文复制引用
宋婷,杭嘉慧,石晓彤,刘兴彩,刘晓霞,麻冬梅..湖南稷子种质资源苗期耐盐性鉴定及耐盐种质筛选[J].中国草地学报,2026,48(4):15-29,15.基金项目
紫花苜蓿耐盐碱基因挖掘及分子机制解析(U24A20426) （U24A20426）
农业生物育种重大项目(2023ZD040600102) （2023ZD040600102）
