中国草地学报2026，Vol.48Issue(4)：68-78,11.DOI:10.16742/j.zgcdxb.20250330
紫花苜蓿MsJUB1基因克隆、亚细胞定位及转录自激活分析
Cloning,Subcellular Localization,and Transcriptional Autoactivation Analysis of MsJUB1 Gene in Alfalfa(Medicago sativa)
摘要
Abstract
JUNGBRUNNEN1(JUB1),a member of the NAC transcription factor family,plays a key role in delaying senescence and mediating responses to stress.However,the function in Medicago sativa remains largely unclear.In this study,the MsJUB1 gene was successfully cloned from the alfalfa cultivar GIB.The open reading frame was 936 bp in length and encoded 311 amino acids.Bioinformatics analysis indicated that the protein was a stable hydrophilic protein lacking both a signal peptide and a transmembrane domain.Analysis of cis acting elements showed that the promotor region of MsJUB1 contained multiple stress and hormone responsive elements,including those associated with defense and stress response,abscisic acid,methyl jasmonate,and gibberellin.Subcellular localization analysis demonstrated that MsJUB1 was localized in the nucleus,and yeast two-hybrid assays confirmed that it possessed transcrip-tional autoactivation activity.RT-qPCR analysis further showed that under NaCl treatment,MsJUB1 expression in the roots,stems and leaves of salt tolerant cultivar GIB initially decreased and then increased significantly with prolonged stress exposure.In contrast,expression in the roots and stems of the salt sensi-tive cultivar LS first increased and then declined,whereas expression in the leaves of LS decreased continu-ously over time.These expression patterns suggested that MsJUB1 was involved in salt stress response in M.sativa.关键词
紫花苜蓿/MsJUB1/基因克隆/亚细胞定位/酵母双杂交Key words
Medicago sativa/MsJUB1/Gene cloning/Subcellular localization/Yeast two-hybrid分类
农业科技引用本文复制引用
吕梦雪,余如刚,韦英铭,刘名江,李昕霞,王国良..紫花苜蓿MsJUB1基因克隆、亚细胞定位及转录自激活分析[J].中国草地学报,2026,48(4):68-78,11.基金项目
财政部和农业农村部:国家现代农业产业技术体系(CARS-34) （CARS-34）
淮北师范大学校级科学研究项目(2024ZK38) （2024ZK38）
山东省基地和人才计划(WSR2023050) （WSR2023050）
