中国防痨杂志2026，Vol.48Issue(5)：707-715,9.DOI:10.19982/j.issn.1000-6621.20250449
基于CRISPR-Cas系统的结核分枝杆菌检测研究进展
Advances in CRISPR-Cas system-based detection of Mycobacterium tuberculosis
摘要
Abstract
Tuberculosis(TB),caused by Mycobacterium tuberculosis(MTB),poses a severe threat to global public health security.However,traditional MTB detection methods,such as smear microscopy and bacterial culture,suffer from insufficient sensitivity,prolonged turnaround times,and delayed drug resistance detection,which have constrained the achievement of the World Health Organization(WHO)'s"End TB"strategy.Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)technology,characterized by high specificity and rapidity,offers transformative potential for MTB testing.This review systematically outlines the latest research progress of CRISPR in MTB point-of-care testing,drug resistance gene detection,and multi-target combined testing.It highlights key challenges in current research,including complex sample pretreatment,poor reagent stability,and inadequate adaptability to primary healthcare settings.Future efforts should focus on simplifying and integrating sample pretreatment and optimizing reagent lyophilization techniques,aiming to provide references for the clinical translation of CRISPR-based MTB detection and the precise prevention and control of TB.关键词
分枝杆菌,结核/CRISPR-Cas系统/诊断技术/分子生物学检测Key words
Mycobacterium tuberculosis/CRISPR-Cas system/Diagnose technology/Molecular diagnostic techniques分类
医药卫生引用本文复制引用
黄震洋,李文君,孙岩松,李浩..基于CRISPR-Cas系统的结核分枝杆菌检测研究进展[J].中国防痨杂志,2026,48(5):707-715,9.基金项目
Capital's Funds for Health Improvement and Research(grant number CFH2024-2-5093) 首都卫生发展科研专项(首发2024-2-5093) （grant number CFH2024-2-5093）
