局解手术学杂志2026，Vol.35Issue(4)：308-315,8.DOI:10.11659/jjssx.05E025091
柴胡皂苷d逆转乳腺癌放疗抵抗的分子机制
Molecular mechanism of saikosaponin-d in reversing radioresistance of breast cancer
摘要
Abstract
Objective To investigate the molecular mechanisms of saikosaponin-d in reversing radioresistance of breast cancer.Methods The potential targets of saikosaponin-d were predicted using online databases,and differentially expressed genes associated with radioresistance of breast cancer were obtained from the GEO database.The intersecting targets were then screened,followed by network topology analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis.The online databases were employed to assess the expression differences of the core targets between normal breast tissue and breast cancer tissue,as well as their prognostic significance.Molecular docking technology was used to verify the interaction between saikosaponin-d and core targets.Results The potential targets of saikosaponin-d in reversing radioresistance of breast cancer mainly included TP53,HSP90AA1,MYC,HSP90AB1,EGFR,H3-3B,JUN,HDAC1,CDK2,TGFB1,mTOR,and RELA,and these targets were enriched in multiple pathways such as PD-1/PD-L1 and AGE-RAGE.The HPA database revealed that MYC,CDK2,and HSP90AB1 were highly expressed in breast cancer tissues,and the Kaplan-Meier Plotter database showed that H3-3B,TGFB1,mTOR and RELA were significantly related to the occurrence and prognosis of breast cancer.Molecular docking technology demonstrated that saikosaponin-d has good binding ability to the core targets.Conclusion Saikosaponin-d may reverse the radioresistance of breast cancer through multiple core targets,including H3-3B,mTOR,and RELA,as well as multiple pathways including PD-1/PD-L1 and AGE-RAGE,thereby providing a new theoretical basis for its clinical application.关键词
柴胡皂苷d/乳腺癌/网络药理学分析/分子对接技术/放疗抵抗Key words
saikosaponin-d/breast cancer/network pharmacology/molecular docking technology/radioresistance分类
医药卫生引用本文复制引用
饶小慧,郑浠雅,罗倩雯,王平汉,王婷,龙方懿..柴胡皂苷d逆转乳腺癌放疗抵抗的分子机制[J].局解手术学杂志,2026,35(4):308-315,8.基金项目
国家自然科学基金面上项目(82374088) （82374088）
四川省科技厅自然科学基金面上项目(2025ZNSFSC0626) （2025ZNSFSC0626）
四川省干部保健科研项目(川干研2023-2201) （川干研2023-2201）
四川省中医药管理局面上项目(2024MS528) （2024MS528）
四川省卫健委青年苗圃项目(24QNMP080) （24QNMP080）
成都市科技局技术创新研发项目(2024-YF05-00744-SN) （2024-YF05-00744-SN）
