中国临床药理学杂志2026，Vol.42Issue(2)：178-184,7.DOI:10.13699/j.cnki.1001-6821.2026.02.005
髓母细胞瘤患儿脑脊液METTL3表达与预后的相关性研究
Expression of METTL3 in cerebrospinal fluids of pediatric patients with medulloblastoma and its correlation with prognosis
摘要
Abstract
Objective To investigate the expression of methyltransferase-like 3(METTL3)in cerebrospinal fluids(CSF)and its correlation with the prognosis of children with medulloblastoma(MB).Methods Cerebrospinal fluids were collected from MB children.METTL3 protein levels were quantified using the Enzyme-Linked ImmunoSorbent Assay(ELISA)and its correlations with clinicopathological features were analyzed.The prognostic significance was evaluated via the Kaplan-Meier survival curve and Cox proportional hazards regression.Bioinformatics analysis was conducted to assess the association of METTL3 with chemotherapeutic drug sensitivities and functional enrichment of METTL3 in brain tumors.Results Ki-67 index was the only clinicopathological factor significantly correlated with CSF METTL3 levels(P＜0.05).METTL3 high expression group showed superior progression-free survival(PFS)(HR=0.376,95％CI:0.133-1.064,P＜0.05).Cox analysis identified METTL3 expression as an independent prognostic factor for PFS(P＜0.05).Significant negative correlations between METTL3 expression and 50％inhibitory concentrations(IC50)of methotrexate/vinblastine/etoposide(P＜0.001).Gene enrichment revealed METTL3's involvement in neural development and Notch signaling pathways.Conclusion METTL3 might serve as a prognostic biomarker for MB pediatric patients.Further validation with expanded cohorts and mechanistic studies(in vivo/in vitro)is warranted.关键词
髓母细胞瘤/甲基转移酶样3/脑脊液/生物标志物/预后Key words
medulloblastoma/methyltransferase-like 3/cerebrospinal fluid/biomarker/prognosis分类
医药卫生引用本文复制引用
刘思涵,李苗,王淑梅..髓母细胞瘤患儿脑脊液METTL3表达与预后的相关性研究[J].中国临床药理学杂志,2026,42(2):178-184,7.基金项目
国家自然科学基金资助项目(No.81872926) （No.81872926）
首都医科大学附属北京世纪坛医院院基金资助项目(No.2023-c01) （No.2023-c01）
