中国临床药理学杂志2026，Vol.42Issue(2)：192-196,5.DOI:10.13699/j.cnki.1001-6821.2026.02.007
屎肠球菌中vanM基因簇表型与基因型相关性研究
Investigation of phenotypic and genotypic correlations of the vanM gene cluster in Enterococcus faecium
苏博 1陈晓林 2郑波1
作者信息
- 1. 北京大学第一医院临床药理研究所,北京 100034
- 2. 北京大学第一医院泌尿外科,北京 100034
- 折叠
摘要
Abstract
Objective To reveal the association between the vanM gene and different glycopeptide resistance phenotypes in Enterococcus faecium,drug susceptibility testing and sequencing analysis were conducted on four E.faecium strains carrying the vanM gene.Methods The broth microdilution method was employed to determine the susceptibility of four E.faecium strains to glycopeptide antibiotics.Polymerase chain reaction(PCR)was performed to detect the vancomycin-resistant genotypes,while population analysis profiling(PAP)was used to identify the presence of heteroresistant strains among vancomycin-susceptible isolates.Quantitative real-time PCR(qRT-PCR)was conducted to analyze the relative expression level of the vanM gene in E.faecium,and genomic sequencing was carried out for alignment of the resistance gene cluster.Results All four E.faecium strains carried the vanM gene.Strain SZD1 was susceptible to vancomycin,whereas SZD2 was identified as a vancomycin-heteroresistant strain.Strain SZD3 exhibited a high relative expression level of the vanM gene and showed resistance to vancomycin.In addition,strain SZD4 harbored both vanA and vanM genotypes,conferring high-level resistance to vancomycin.Conclusion E.faecium strains harboring the vanM gene can exhibit diverse glycopeptide resistance phenotypes,and such variations may be associated with the structure,copy number,and expression level of the vanM gene cluster,as well as the coexistence of other resistance genes.关键词
万古霉素/替考拉宁/屎肠球菌/vanM基因簇Key words
vancomycin/teicoplanin/Enterococcus faecium/vanM gene cluster分类
医药卫生引用本文复制引用
苏博,陈晓林,郑波..屎肠球菌中vanM基因簇表型与基因型相关性研究[J].中国临床药理学杂志,2026,42(2):192-196,5.
