中国临床医学2026，Vol.33Issue(2)：277-284,8.DOI:10.12025/j.issn.1008-6358.2026.20251366
酸枣仁皂苷B对急性癫痫发作的缓解及神经保护作用
Anticonvulsant and neuroprotective effects of jujuboside B on acute epilepsy
摘要
Abstract
Objective To investigate the alleviating effect of jujuboside B(JuB)on acute epilepsy in mice and its protective effect on hippocampal neurons.Methods An in vitro epilepsy model was established by stimulating primary hippocampal neurons with cyclothiazide(CTZ).Spontaneous epileptiform discharge was recorded using patch clamp technique.An acute epilepsy model was induced in adult male C57BL/6 mice by intraperitoneal injecting pentylenetetrazol(PTZ)following pretreatment with JuB,and severity of epilepsy was recorded.Mice were euthanized and brain tissues were collected 24 hours after model establishment.The expression levels of mitoptosis related proteins in the hippocampus were detected by Western blotting.Neuronal damage in the hippocampal CA1 and CA3 regions was observed using pathological staining.Results JuB reduced the frequency of CTZ-induced epileptiform discharges(P＜0.001).Pretreatment with 30 mg/kg and 50 mg/kg JuB decreased the maximal behavioral seizure score and prolonged the latency to Racine stage Ⅲ seizures in PTZ-induced epileptic mice(P＜0.001).Compared with the PTZ group,JuB treatment downregulated Bax protein level(P＜0.01)and upregulated Bcl-2 protein level(P＜0.05)in acute epileptic mice.Furthermore,JuB protected neuronal viability in the hippocampal CA1(P＜0.05)and CA3(P＜0.01)regions,and ameliorated pathological morphological changes including cellular disarray,unclear boundaries,pyknosis,fragmentation,and dissolution.Conclusions JuB exhibits antiepileptic effects both in vivo and in vitro.It exerts potential antiepileptic effects by inhibiting mitoptosis and attenuating neuronal damage in the hippocampus in an acute epilepsy model.关键词
急性癫痫/海马/线粒体凋亡/酸枣仁皂苷BKey words
acute epilepsy/hippocampus/mitoptosis/jujuboside B分类
医药卫生引用本文复制引用
韩婉若,支琳钰,Benjamin Hongye Wang,刘旭..酸枣仁皂苷B对急性癫痫发作的缓解及神经保护作用[J].中国临床医学,2026,33(2):277-284,8.基金项目
国家自然科学基金(81971204).Supported by National Natural Science Foundation of China(81971204). （81971204）
