中国免疫学杂志2026，Vol.42Issue(4)：788-793,6.DOI:10.3969/j.issn.1000-484X.2026.04.004
miR-29a-3p靶向调控FSTL1对肺炎链球菌诱导的肺泡上皮细胞损伤的影响
Effect of miR-29a-3p on Streptococcus pneumonia induced alveolar epithelial cell injury by target regulating FSTL1
摘要
Abstract
Objective:To investigate the effect of miR-29a-3p on alveolar epithelial cell injury induced by Streptococcus pneumonia(SP)by regulating follistatin-like protein 1(FSTL1).Methods:HPAEpiC cells were divided into control(Ctrl)group,SP group,SP+miR-NC group,SP+miR-29a-3p mimic group,SP+miR-29a-3p mimic+OE-NC group,and SP+miR-29a-3p mimic+FSTL1-OE group.qRT-PCR was used to detect levels of miR-29a-3p and FSTL1 mRNA in cells.CCK-8 methods was used to determine prolif-eration ability of cells in each group.Annexin Ⅴ-PI method was used to detect level of apoptosis of cells in each group.ELISA was used to detect levels of TNF-α and IL-6 of cells in each group.SOD activity and the amount of ROS of cells in each group were detected by kits.Western blot was used to detect expression levels of FSTL1,Bax and Bcl-2 proteins of cells in each group.Dual luciferase assay was used to determine the targeting relationship between miR-29a-3p and FSTL1.Results:Compared with Ctrl group,proliferation inhibition rate,apoptosis rate,TNF-α,IL-6,ROS,Bax and FSTL1 mRNA and protein expression levels were significantly higher in SP group,SP+miR-NC group,SP+miR-29a-3p mimic group,SP+miR-29a-3p mimic+OE-NC group,SP+miR-29a-3p mimic+FSTL1-OE group(P<0.05),while miR-29a-3p level,cell survival rate,SOD and Bcl-2 levels were significantly lower(P<0.05).Compared with SP+miR-NC group,proliferation inhibition rate,apoptosis rate,TNF-α,IL-6,ROS,Bax and FSTL1 mRNA and protein expression levels were significantly lower in SP+miR-29a-3p mimic group(P<0.05),while miR-29a-3p level,cell survival rate,SOD and Bcl-2 levels were significantly higher(P<0.05).Compared with SP+miR-29a-3p mimic+OE-NC group,proliferation inhibition rate,apoptosis rate,TNF-α,IL-6,ROS,Bax,and FSTL1 mRNA and protein expression levels were significantly higher in SP+miR-29a-3p mimic+FSTL1-OE group(P<0.05),while miR-29a-3p level,cell survival rate,SOD and Bcl-2 levels were significantly lower(P<0.05).Compared with FSTL1-WT and miR-NC co-transfected cells,fluorescence enzyme activity in FSTL1-WT and miR-29a-3p mimic co-transfected group was significantly lower(P<0.05).Conclusion:miR-29a-3p may alleviate SP-induced alveolar epithelial cell injury,promote cell proliferation,inhibit cell apoptosis and inflammatory response by target down-regulating FSTL1.关键词
miR-29a-3p/卵泡蛋白样蛋白1/肺炎链球菌/肺泡上皮细胞损伤Key words
miR-29a-3p/Follistatin-like protein 1/Streptococcus pneumonia/Alveolar epithelial cells injury分类
医药卫生引用本文复制引用
王莲,杜婧慧,孙寅,蔡令辉..miR-29a-3p靶向调控FSTL1对肺炎链球菌诱导的肺泡上皮细胞损伤的影响[J].中国免疫学杂志,2026,42(4):788-793,6.基金项目
山东省医药卫生科技发展计划项目(202006010355). （202006010355）
