中国免疫学杂志2026，Vol.42Issue(4)：794-800,807,8.DOI:10.3969/j.issn.1000-484X.2026.04.005
雷公藤多苷对过敏性紫癜肾炎大鼠的影响机制研究
Mechanism of effect of tripterygium wilfordii glycosides on rats with henoch-schonlein purpura nephritis
摘要
Abstract
Objective:To investigate the protective effect of tripterygium wilfordii glycosides(GTW)on kidney in rats with henoch-schonlein purpura nephritis(HSPN),and its regulation on polarization of M1/M2 macrophages mediated by Toll-like receptor 4(TLR4)signaling pathway.Methods:Rat model of HSPN was established by intragastric administration of bovine serum albumin(BSA)+LPS+carbon tetrachloride(CCl4),supplemented with dried ginger water.Experimental groups were as follows:control group,model group(HSPN),GTW low dose group(GTW-L),GTW high dose group(GTW-H),agonist group(LPS).Automatic biochemi-cal instrument was used to detect 24-hour urinary protein and renal function.ELISA was used to determine contents of IL-6 and TNF-α in serum of rats in each group.TUNEL staining was used to detect apoptosis rate in renal tissue,immunohistochemistry was used to de-tect the polarization of macrophages in rat renal tissue.Western blot was used to detect expressions of TLR4,myeloid differentiation factor 88(MyD88),NF-κB,p-NF-κB,Bcl-2 and Bax.Results:GTW could significantly reduce the quantity of 24-hour urinary pro-tein,improve renal function,decrease contents of IL-6 and TNF-α in serum,down-regulate rate of apoptosis in renal tissue,inhibit sig-nal transduction of TLR4,decrease expressions of TLR4,MyD88,NF-κB and Bax,increase expression of Bcl-2,while LPS could partially reverse the protective effect of GTW on renal tissue,and the differences were statistically significant(all P<0.05).Conclu-sion:GTW can significantly inhibit expressions of inflammatory mediators in renal tissue of HSPN rats,reduce apoptosis rate of renal tissue,reduce the pathological injury of renal tissue,and improve the renal function of rats,which may be related to its ability to inhibit the expression of TLR4 signaling pathway and promote the conversion of renal macrophages to type M2.关键词
雷公藤多苷/过敏性紫癜肾炎/Toll样受体4信号通路/巨噬细胞极化Key words
Tripterygium wilfordii glycosides/Henoch-schonlein purpura nephritis/Toll-like receptor 4 signaling pathway/Macrophage polarization分类
医药卫生引用本文复制引用
郑晓娟..雷公藤多苷对过敏性紫癜肾炎大鼠的影响机制研究[J].中国免疫学杂志,2026,42(4):794-800,807,8.基金项目
河南省中医药传承与创新第二批拔尖人才培养项目(豫卫中医函[2021]15号) （豫卫中医函[2021]15号）
河南省中医药科学研究专项课题(2023ZY2157). （2023ZY2157）
