Abstract
Objective:To explore the effect of Huoxue Zishen Decoction on helper T cell 17/regulatory T cell(Th17/Treg)bal-ance and the expression of inflammatory factors in endometriosis(EMs)rats.Methods:A total of 60 rats were randomly divided into Sham group,EMs group,low-dose Huoxue Zishen Decoction(HXZS-L)group,and high-dose Huoxue Zishen Decoction(HXZS-H)group,with 15 rats in each group.Rat models of EMs were established by autologous loendometrial transplantation.Rats in Sham group only underwent ligation and resection of the left uterine horn,without transplantation.After 2 weeks of modeling,rats in HXZS-L group and HXZS-H group were orally administered with Huoxue Zishen Decoction(10.71 g/kg,42.84 g/kg)daily;rats in Sham group and EMs group were orally administered an equal amount of physiological saline for 3 consecutive weeks.After 3 weeks of treat-ment,ELISA was used to detect inflammatory factors IL-6,IL-17A,IL-22,IL-10 and TGF-β levels in serum;HE staining was used to observe ectopic endometrial tissue damage;flow cytometry was used to detect Th17/Treg in peripheral blood;RT-qPCR,Western blot were used to detect Th17/Treg related markers(IL-17A,RORγt,Foxp3,GITR,CTLA-4)mRNA and protein levels in ectopic endometrial tissue.Results:Compared with Sham group,the endometrium lesions of rats in EMs group were raised and transparent,with a large number of blood vessels covering the surface,a large number of blood vessels were formed around,and ectopic endometrial epithelial cells were arranged in a multi-layered disordered manner,the proportion of Th17 and Th17/Treg in peripheral blood were in-creased,the proportion of Treg was decreased,and the IL-17A,RORγt mRNA and protein levels in ectopic endometrial tissue were increased,while Foxp3,GITR,CTLA-4 mRNA and protein levels were decreased,IL-6,IL-17A,and IL-22 levels in serum were in-creased,IL-10,TGF-β levels in serum were decreased(P<0.05);compared with EMs group,the ectopic endometrial lesions of rats in HXZS-L group and HXZS-H group were reduced,with a small number of blood vessels were formed around,and ectopic endometrial epithelial cells were arranged in a single layer in an orderly manner,the proportion of Th17 and Th17/Treg in peripheral blood were de-creased,the proportion of Treg was increased,and the IL-17A,RORγt mRNA and protein levels in ectopic endometrial tissue were decreased,while Foxp3,GITR,CTLA-4 mRNA and protein levels were increased,IL-6,IL-17A and IL-22 levels in serum were de-creased,IL-10,TGF-β levels in serum were increased(P<0.05);and the effect of HXZS-H group was better than that of HXZS-L group(P<0.05).Conclusion:Huoxue Zishen Decoction could regulate balance of Th17/Treg and the expression of related inflammato-ry factors in EMs rats,and inhibit the growth of ectopic lesions.关键词
活血滋肾方/子宫内膜异位症/Th17/Treg/炎症因子Key words
Huoxue Zishen Decoction/Endometriosis/Th17/Treg/Inflammatory factors分类
医药卫生
