中国农学通报2026，Vol.42Issue(8)：183-190,8.
油菜根系分泌物对4种农田入侵杂草的化感作用研究
Allelopathic Effects of Brassica juncea(YRab2)Root Exudates on Four Invasive Weeds
摘要
Abstract
This study aims to evaluate the allelopathic potential of Brassica juncea(YRab2)against dominant invasive weeds in agricultural systems,thereby providing a scientific foundation for the green and sustainable management of invasive weed species.This study employed the Petri dish filter paper method to assess the allelopathic effects of root exudates from Brassica juncea(YRab2)on seed germination and seedling growth of four invasive weed species:Phalaris minor,Avena fatua,Galinsoga parviflora,and Ageratum conyzoides.The allelopathic index(RI),comprehensive allelopathic effect(SE),and toxicity(LC50)values,were quantitatively evaluated.The results showed that within the concentration range of 5 to 80 mL/plant,the root exudates of Brassica juncea(YRab2)significantly inhibited seed germination and seedling growth of the four invasive weed species.The allelopathic response,assessed through the relative inhibition index(RI),showed negative values across all measured parameters,including germination rate(RI:-0.621 to-0.008),germination index(RI:-0.844 to-0.039),root length(RI:-0.865 to-0.056),shoot length(RI:-0.824 to-0.021),and biomass(RI:-0.734 to-0.063),indicating consistent inhibitory effects.The comprehensive allelopathic effect(SE)also remained negative(range:-0.761 to-0.040).Notably,the magnitude of inhibition increased with decreasing concentration,suggesting a non-monotonic dose-response relationship.At concentrations of 5,10,20,and 40 mL/plant,all parameters were significantly suppressed compared to the control(P<0.05),with the strongest inhibition observed at the lowest tested concentration(5 mL/plant).These results demonstrate that Brassica juncea(YRab2)root exudates exert a potent allelopathic inhibitory effect on both seed germination and early seedling development of the target weeds.Toxicity analysis revealed that the root exudates of v Brassica juncea(YRab2)exhibited differential inhibitory effects on seed germination and seedling growth across the four invasive weed species.The lowest LC50 values,indicative of higher phytotoxicity,were observed for Galinsoga parviflora seed germination(LC50:8.023 mL/plant for germination rate;19.979 mL/plant for germination index)and Ageratum conyzoides seedling growth(LC50:21.292 mL/plant for root length;20.316 mL/plant for shoot length),suggesting greater sensitivity of these species to the exudates compared to others.In contrast,Avena fatua displayed the highest LC50 values,indicating the lowest susceptibility to both germination and growth inhibition.These findings demonstrate that,under equivalent concentrations of root exudate application,Galinsoga parviflora and Ageratum conyzoides experience more pronounced inhibitory effects,particularly in early developmental stages.The root exudates of Brassica juncea(YRab2)exhibit strong allelopathic inhibitory potential against both seed germination and seedling growth of four invasive weed species.Notably,they show pronounced phytotoxic effects on Galinsoga parviflora and Ageratum conyzoides,highlighting their promise for application in ecological weed management and sustainable,non-chemical control strategies.These findings provide a scientific foundation for the green and environmentally sustainable governance of invasive weeds in agricultural systems.关键词
芥菜型油菜/根系分泌物/化感作用/种子萌发/幼苗生长/毒力Key words
Brassica juncea/root exudates/allelopathy/seed germination/seedling growth/toxicity分类
农业科技引用本文复制引用
刘雪玲,孔燕,袁静静,闫培睿,闫茂鹏,徐高峰,梁博铨,姚彬,杨云海,展澈,申时才,张付斗,杨韶松,郑凤萍..油菜根系分泌物对4种农田入侵杂草的化感作用研究[J].中国农学通报,2026,42(8):183-190,8.基金项目
云南省农业联合专项重点项目"油菜根系分泌物对外来入侵植物小子虉草种子休眠与萌发的调控机制"(202301BD070001-030) （202301BD070001-030）
国家自然科学基金项目"生态控制过程中薇甘菊功能性状应对气候变暖的响应机制及对其种群扩展的调控"(32260302) （32260302）
云南省专家基层科研工作站项目"芒市土壤肥料工作站徐高峰专家基层科研工作站"(20250244). （20250244）
