中国农业大学学报2026，Vol.31Issue(6)：195-205,11.DOI:10.11841/j.issn.1007-4333.2026.06.16
重度低氧对小鼠肝脏成纤维细胞生长和线粒体功能的影响
Effect of acute sever hypoxia on the growth and mitochondrial function of the mouse liver fibroblasts
摘要
Abstract
To investigate the mechanisms of hypoxia-induced cellular injury,mouse liver fibroblasts were exposed to three representative high-altitude hypoxic conditions,including 11％O2 representing mild hypoxia,5％O2 representing moderate hypoxia,and 1％O2 representing severe hypoxia.Cell viability was assessed using the MTT assay,ultrastructural changes were examined by transmission electron microscopy,intracellular oxidative stress was evaluated by ROS fluorescent probing,and mitochondrial membrane potential was assessed by JC-1 staining.In addition,RT-qPCR and Western blot were performed to determine the transcriptional and protein expression levels of genes related to mitochondrial function and apoptosis.Compared with the normoxic control group(21％O2),11％O2 treatment caused no obvious changes in cell density or morphology,and cell viability showed a time-dependent increasing trend without statistical significance(P＞0.05),whereas 5％and 1％O2 treatments induced marked morphological alterations.Under 1％O2 exposure,cell viability did not differ significantly from the normoxic control at 24 h(P＞0.05)but declined sharply at 48 h and 72 h(P＜0.001),reaching the lowest level at 72 h.This reduction was accompanied by increased ROS accumulation,loss of mitochondrial membrane potential,and pronounced mitochondrial ultrastructural damage,including mitochondrial swelling and cristae disruption.Furthermore,after 72 h of 1％O2 treatment,the Bax/Bcl-2 ratio was significantly increased at both mRNA and protein levels(P＜0.05),while the mitochondrial fission-related gene Drp1 was significantly downregulated at both transcriptional and protein levels(P＜0.05).Collectively,severe hypoxia at 1％O2 impaired mitochondrial integrity and function,promoted apoptosis,and reduced cell viability,indicating that profound hypoxia induces hypoxia-associated injury in mouse liver fibroblasts.关键词
重度低氧/细胞活力/线粒体/凋亡/肝脏成纤维细胞Key words
sever hypoxia/cell viability/mitochondrion/apoptosis/liver fibroblast cells分类
农业科技引用本文复制引用
马小娟,陈功,杨雨泽,张聪聪,侯玲玲,窦金焕,徐青..重度低氧对小鼠肝脏成纤维细胞生长和线粒体功能的影响[J].中国农业大学学报,2026,31(6):195-205,11.基金项目
红果园-国家重点研发计划项目(V23GY1200011) （V23GY1200011）
国家自然科学基金青年科学基金项目(32202644) （32202644）
