中国全科医学2026，Vol.29Issue(18)：2504-2512,9.DOI:10.12114/j.issn.1007-9572.2025.0137
腹部动力灸对围绝经期失眠大鼠单胺类神经递质及脑肠肽的影响研究
Effect of Abdominal Dynamic Moxibustion on Monoamine Neurotransmitters and Brain-gut Peptides in Perimenopausal Insomnia Rats
摘要
Abstract
Background Perimenopausal insomnia adversely affects women's health.Western medicine primarily employs hormone replacement therapy(HRT)and sedative-hypnotic drugs for its treatment.However,HRT is associated with a high recurrence rate and carries increased risks of thrombosis and carcinogenesis,while sedative-hypnotics often lead to issues of dependence and addiction.Therefore,exploring safe and effective non-pharmacological treatments holds significant clinical importance.Objective To investigate the potential mechanism of action of dynamic moxibustion in treating perimenopausal insomnia.Methods Forty SPF-grade adult female SD rats were randomly divided into a sham-operated group,a model group,a dynamic moxibustion group,a suspended moxibustion group,and a positive control group using a block randomization method,with 8 rats per group.A rat model of perimenopausal insomnia was established.Rats in the sham-operated and model groups received intragastric administration of 0.9%NaCl solution(10 mL/kg).The dynamic moxibustion group received treatment with dynamic moxibustion.The suspended moxibustion group received intervention with circling moxibustion.The positive control group received intragastric administration of 0.1 mg/L estazolam solution(10 mL/kg),once daily.All interventions lasted for 15 consecutive days.One week after ovariectomy,vaginal exfoliated cells were collected for Giemsa staining.After the intervention period,a pentobarbital sodium cooperative sleep test was conducted to evaluate sleep duration.The Morris water maze test was used to assess learning and memory abilities in the perimenopausal rat model.After the completion of the Morris water maze and pentobarbital sodium tests,rats were anesthetized and sacrificed,and hypothalamic and colon tissues were collected.Hematoxylin-eosin(HE)staining was performed to observe pathological changes in colon tissue.Immunohistochemical staining was used to detect the protein expression of substance P(SP)and neuropeptide Y(NPY)in the hypothalamus and colon tissues.Western blot analysis was used to detect SP and NPY protein expression in thalamic tissue.Enzyme-linked immunosorbent assay(ELISA)was used to measure serum levels of SP,NPY,5-hydroxytryptamine(5-HT),norepinephrine(NE)and estradiol(E2).Results Giemsa staining results showed that rats in the sham-operated group maintained a regular estrous cycle,while the modeling success rate,indicated by loss of the estrous cycle,was 100%in the model group,suggesting the rats entered a perimenopausal state.Compared with the sham-operated group,the model group exhibited prolonged sleep latency and shortened sleep duration.Compared with the model group,all treatment groups showed shortened sleep latency and prolonged sleep duration.Compared with the suspended moxibustion group,the dynamic moxibustion group demonstrated shortened sleep latency and significantly prolonged sleep duration(P<0.05).Morris water maze results indicated that compared with the sham-operated group,the model group showed decreased platform crossings,prolonged escape latency,and increased total path length.Compared with the model group,all intervention groups exhibited a shortened total path length,decreased escape latency,and increased platform crossings(P<0.05).HE staining revealed that the sham-operated group had regular,intact,and orderly arranged colonic tissue structure with abundant neurons.The model group showed irregular colonic tissue surfaces,structural changes,and a decreased number of neuronal cells.Compared with the model group,all intervention groups demonstrated more uniformly and orderly arranged colonic crypts and an increased number of neuronal cells,with the abdominal dynamic moxibustion group showing marked improvement.Immunohistochemical staining results showed that compared with the sham-operated group,the model group had decreased protein expression of SP and NPY in the colon and thalamic tissues(P<0.05).Compared with the model group,all intervention groups showed increased protein expression of SP and NPY in the colon and thalamic tissues(P<0.05).Compared with the suspended moxibustion group,the dynamic moxibustion and positive control groups exhibited increased protein expression of SP and NPY in the colon and thalamic tissues(P<0.05).Western blot results indicated that compared with the sham-operated group,the model group had increased protein expression of SP and NPY in the thalamic tissue(P<0.05).Compared with the model group,all intervention groups showed decreased protein expression of SP and NPY in the thalamic tissue(P<0.05).Compared with the suspended moxibustion group,the dynamic moxibustion and positive control groups exhibited increased SP protein expression(P<0.05).ELISA results demonstrated that compared with the sham-operated group,the model group had significantly decreased serum levels of SP,NPY,5-HT,and E2,and significantly increased NE levels.Compared with the model group,all intervention groups showed significantly increased serum levels of SP,NPY,and 5-HT,and decreased NE levels.Compared with the suspended moxibustion group,the dynamic moxibustion group showed significantly increased serum SP and 5-HT levels,and decreased NE levels(P<0.05).Conclusion Abdominal dynamic moxibustion can improve sleep and spatial cognitive ability in a rat model of perimenopausal insomnia.Its mechanism of action may be associated with the regulation of brain-gut peptide and monoamine neurotransmitter expression.关键词
围绝经期失眠/动力灸/脑肠肽/单胺类神经递质/雌二醇Key words
Perimenopausal insomnia/Dynamic moxibustion/Brain-gut peptide/Monoamine neurotransmitter/Estradiol分类
医药卫生引用本文复制引用
张晓婷,张雪枫,邱思淦,刘俊昌,古来撒尔·艾克拜尔,卢旭昇,樊思恩,康霞,兰永利..腹部动力灸对围绝经期失眠大鼠单胺类神经递质及脑肠肽的影响研究[J].中国全科医学,2026,29(18):2504-2512,9.基金项目
新疆维吾尔自治区自然科学基金面上项目(2022D01C199) （2022D01C199）
国家自然科学基金地区项目(81960906) （81960906）
