中国蔬菜Issue(4)：149-156,8.DOI:10.19928/j.cnki.1000-6346.2026.5008
新德里番茄曲叶病毒温州番茄分离物分子鉴定
Molecular Analysis of Isolates of Tomato Leaf Curl New Delhi Virus Collected from Wenzhou Tomato
摘要
Abstract
Samples of land tomato with curled and obviously dwarfed leaves were collected from Wenzhou,Zhejiang Province,China.In order to clarify the pathogenic species,RNA of the disease samples was extracted,reverse transcribed into cDNA,and amplified by RT-PCR with the Geminiviridae viral universal primers PA/PB,and its amplified product was identified by sequencing as tomato leaf curl New Delhi virus(ToLCNDV).In order to clarify the evolutionary status of the Wenzhou isolate of tomato leaf curl New Delhi virus,the whole genome sequences of viral DNA-A and DNA-B were obtained by RT-PCR using virus-specific primers.Phylogenetic analysis was performed using Tbtools software,and the results showed that the tomato leaf curl New Delhi virus Wenzhou isolate clustered with the Chinese isolates,which was closest in affinity,and distant from the foreign isolates.The use of virus-encoded proteins to construct transgenic plants is an effective measure to prevent and control viruses.In this study,the genes of AV1(encoding the capsid protein)and BV1(encoding the nucleus shuttling protein)of the Wenzhou isolate of tomato leaf curl New Delhi virus were further cloned,and the transient expression of the tobacco showed that AV1-YFP and BV1-YFP were localized in the nucleus.The results of this study will provide experimental data for further understanding of the distribution of tomato leaf curl New Delhi virus in China,and lay a molecular foundation for the use of virus-encoded proteins to construct transgenic plants for virus prevention and control.关键词
新德里番茄曲叶病毒/番茄/基因组序列/细胞定位Key words
tomato leaf curl New Delhi virus/tomato/genomic sequences/cell localization引用本文复制引用
陈官菊,梁秀美,荀倩,杨柳,郭秀珠,孙枫,夏海涛..新德里番茄曲叶病毒温州番茄分离物分子鉴定[J].中国蔬菜,2026,(4):149-156,8.基金项目
瓯海科创中心项目(2022OHKC0008),温州市高水平创新团队"沿海特色植物创新和利用团队"项目(NY202401),江苏省自然科学基金面上项目(BK20231386) （2022OHKC0008）
