中国输血杂志2026，Vol.39Issue(4)：493-500,8.DOI:10.13303/j.cjbt.issn.1004-549x.2026.04.011
无创胎儿M血型抗原实时荧光定量PCR基因分型方法的建立及初步评价
Establishment and preliminary evaluation of a non-invasive fetal M blood group genotyping method by real-time PCR
摘要
Abstract
Objective To establish a method for the genotyping of fetal M blood group antigen by extracting cell-free fe-tal DNA(cff-DNA)from maternal plasma,so as to guide the management of M antigen-negative pregnant women with IgG anti-M antibody during pregnancy.Methods A realtime fluorescent quantitative PCR(realtime PCR)method was estab-lished.The specificity and sensitivity of the method were validated by dilution of genomic DNA.Subsequently,a total of 12 M antigen-negative pregnant women were enrolled.The cff-DNA was extracted from maternal plasma,and fetal M antigen genotyping was performed by realtime PCR.Fetuses were classified as M-positive or M-negative according to the presence or absence of amplification curve.The accuracy of the method was validated by comparing fetal M antigen genotyping results with the serological results using the cord or peripheral blood of the neonate at birth.Results Among the 12 M antigen-negative pregnant women,anti-M was detected in five cases,of which four cases had IgG anti-M,and one case had fetal a-nemia.The results of fetal M antigen genotyping showed that 9 cases were M-positive(9/12,75%)and 3 cases were M-negative(3/12,25%).Serological results of blood samples collected after birth from four M-positive fetuses and one M-negative fetus were consistent with the genotyping results.Conclusion We have,for the first time,established a non-inva-sive prenatal genotyping method for fetal M antigen using maternal plasma cff-DNA,and preliminarily demonstrated the fea-sibility of this method.关键词
抗-M胎儿新生儿溶血病/胎儿游离DNA/无创胎儿M抗原基因检测/实时荧光定量PCR方法Key words
anti-M related hemolytic disease of the fetus and newborn(HDFN)/cell-free fetal DNA(cff-DNA)/non-invasive fetal M antigen genotyping/real-time fluorescent quantitative PCR分类
医药卫生引用本文复制引用
贾双双,莫春妍,魏玲,温机智,张润青,姬艳丽..无创胎儿M血型抗原实时荧光定量PCR基因分型方法的建立及初步评价[J].中国输血杂志,2026,39(4):493-500,8.基金项目
广东省医学科学技术研究项目(B2023201) （B2023201）
广州市科技计划项目(2025A03J3449) （2025A03J3449）
广州市医学重点学科(2025-2027). （2025-2027）
