中国实验方剂学杂志2026，Vol.32Issue(8)：82-88,7.DOI:10.13422/j.cnki.syfjx.20252209
藁本内酯对脑缺血再灌注大鼠中性粒细胞胞外诱捕网的影响
Effect of Ligustilide on Neutrophil Extracellular Traps in Rats with Cerebral Ischemia-reperfusion Injury
摘要
Abstract
Objective:To explore the possible mechanisms by which ligustilide(LIG)exerts neuroprotective effects on ischemic stroke(IS)by inhibiting the release of neutrophil extracellular traps(NETs),promoting blood-brain barrier repair,and alleviating post-ischemic neuroinflammation,thereby providing a new direction for IS treatment.Methods:A middle cerebral artery occlusion(MCAO)model was established in rats.The rats were divided into the sham operation(Sham)group,model(Model)group,low-and high-dose LIG groups(20,40 mg·kg-1),and the NET inhibitor CI-amidine group(CI-amidine,10 mg·kg-1).Drug treatments were administered for 3 days.Neurological injury after ischemia was evaluated by 2,3,5-triphenyltetrazolium chloride(TTC)staining,neurological deficit scoring,and brain index measurement.Flow cytometry and Western blot were used to analyze changes in neutrophil expression.Immunofluorescence was used to observe the fluorescence intensity of the NET marker citrullinated histone H3(H3Cit).Western blot was performed to detect the expression of blood-brain barrier tight junction-related proteins and inflammatory factors,including interleukin-18(IL-18)and interleukin-1β(IL-1β).Results:Compared with the Sham group,the Model group exhibited significant brain tissue injury(P＜0.05),significantly increased neutrophil numbers and NET expression(P＜0.05),significantly impaired blood-brain barrier permeability(P＜0.05),and significantly increased expression of inflammatory factors(P＜0.05).Compared with the Model group,both low-and high-dose LIG significantly alleviated brain tissue injury in rats(P＜0.01),inhibited neutrophil numbers and NET expression(P＜0.01),reduced blood-brain barrier damage(P＜0.01),and suppressed the expression of inflammatory factors IL-18 and IL-1β(P＜0.01),thereby ultimately exerting a neuroprotective effect.Conclusion:The neuroprotective effect of LIG in rats with cerebral ischemia-reperfusion injury may be related to inhibition of neutrophils and the NETs induced by them.关键词
缺血性脑卒中/藁本内酯/中性粒细胞/胞外诱捕网(NETs)/炎症Key words
ischemic stroke/ligustilide/neutrophils/neutrophil extracellular traps(NETs)/inflammation分类
医药卫生引用本文复制引用
吴倩,汪杨,周家宁,万祉含,胡可,黄琪,汪宁..藁本内酯对脑缺血再灌注大鼠中性粒细胞胞外诱捕网的影响[J].中国实验方剂学杂志,2026,32(8):82-88,7.基金项目
国家自然科学基金项目(81903954,81773933) （81903954,81773933）
安徽高校自然科学研究重点项目(2024AH050964,KJ2020A0410) （2024AH050964,KJ2020A0410）
安徽省教育厅青年教师培育项目(YQYB2023010) （YQYB2023010）
中药复方防治脑病的应用基础研究安徽省高校优秀科研创新团队项目(2022AH010034) （2022AH010034）
安徽省高校科研计划项目团队项目(2022AH010036) （2022AH010036）
