中国兽医科学2026，Vol.56Issue(4)：427-434,8.DOI:10.16656/j.issn.1673-4696.2026.0079
PP2对新城疫病毒体外抑制作用的研究
Inhibitory effect of PP2 on Newcastle disease virusin vitro
摘要
Abstract
To evaluate the impact of the Src family tyrosine kinase(SFKs)inhibitor PP2 on Newcastle disease virus(NDV)infection,this study used chicken macrophage HD11 cells as a model and determined the working concentration of PP2 in HD11 cells by assessing cell viability.Subsequently,indirect immunofluorescence,Western-blot,reverse-transcription fluorescent quantitative PCR(RT-qPCR)and hemagglutination assays were employed to assess the effects of PP2 treatment on different stages of NDV infection.The results showed that PP2 significantly inhibited protein expression and progeny virus release of NDV in HD11 cells.Further investigation revealed that PP2 did not affect the adsorption and entry process of NDV,but suppressed the RNA synthesis step that occurred after viral entry.Moreover,PP2 exhibited anti-NDV activity in multiple cell types.Additionally,the SFKs inhibitors PP1 and RK24466,which share the same target as PP2,exhibit comparable anti-NDV efficacy in vitro.In summary,PP2 could effectively inhibit NDV replication.This study provides insights for host-targeted antiviral strategies against NDV.关键词
新城疫病毒/PP2/SFKs/体外抑制作用Key words
Newcastle disease virus/PP2/SFKs/in vitro inhibition分类
农业科技引用本文复制引用
孟子程,师乾凯,韩宗玺,李慧昕,邵昱昊,孙军峰,刘胜旺..PP2对新城疫病毒体外抑制作用的研究[J].中国兽医科学,2026,56(4):427-434,8.基金项目
中央引导地方科技发展专项(ZY04JD03-3) （ZY04JD03-3）
国家蛋鸡产业体系技术岗位科学家项目(CARS-40-K18) （CARS-40-K18）
2024年度博士后创新人才支持计划项目(BX20240422) （BX20240422）
