中国兽医科学2026，Vol.56Issue(4)：520-525,6.DOI:10.16656/j.issn.1673-4696.2026.0058
犬白介素31蛋白表达及其单克隆抗体制备
Expression of canine IL-31 protein and preparation of its monoclonal antibodies
摘要
Abstract
Interleukin-31(IL-31)exerts a key biological role in various cutaneous inflammatory processes by binding to its receptor.In this study,the prokaryotic expression vector for canine IL-31 was constructed,and the recombinant canine IL-31 protein with high purity was successfully expressed and purified using an E.coli expression system.After immunizing BALB/c mice with this recombinant protein,followed by three rounds of immunization,cell fusion,and screening via the limited dilution method,one hybridoma cell line 1F2 that could stably secrete canine IL-31 monoclonal antibody was successfully obtained.The antibody was of the IgG1/λ isotype,and the titer of the purified antibody could reach 1∶13 107 200.To further verify the antibody specificity,a eukaryotic expression plasmid was construct-ed and used to express canine IL-31 in HEK-293T cells.Western-blot results showed that the 1F2 mono-clonal antibody could specifically recognize canine IL-31 protein expressed in the eukaryotic system,in-dicating excellent reaction specificity.The successfully prepared anti-canine IL-31 monoclonal antibody 1F2 in this study lays a foundation for the subsequent development of therapeutic antibodies targeting canine IL-31 and the establishment of related detection methods.关键词
犬IL-31/原核表达/单克隆抗体Key words
canine IL-31/prokaryotic expression/monoclonal antibody分类
农业科技引用本文复制引用
蒋乐凡,刘权,赵成龙,陈兴祥..犬白介素31蛋白表达及其单克隆抗体制备[J].中国兽医科学,2026,56(4):520-525,6.基金项目
国家重点研发计划项目(2023YFD1800302) （2023YFD1800302）
国家自然科学基金面上项目(32273081) （32273081）
