中国糖料2026，Vol.48Issue(2)：1-12,12.DOI:10.13570/j.cnki.scc.2026.02.001
重离子束辐照诱导甘蔗易脱叶突变体的转录组学分析及关键候选基因挖掘
Transcriptomic Analysis and Key Candidate Gene Identification of Leaf Abscission Sugarcane Mutants Induced by Heavy Ion Beam Irradiation
摘要
Abstract
[Objective]This study aimed to investigate the effects of heavy ion beam irradiation-induced mutagenesis on gene expression and functional enrichment in abscission-prone sugarcane mutants,so as to elucidate the molecular mechanisms underlying sugarcane leaf abscission induced by heavy ion beam irradiation.[Methods]The abscission zone tissues of the abscission-prone sugarcane mutant(FS30)and its wild-type(Guitang 42,GT42)were used as experimental materials.Transcriptome sequencing and bioinformatics analysis were performed to identify differentially expressed genes(DEGs)and enriched pathways.[Results]DEGs are predominantly enriched in GO terms related to cellular processes,protein complexes,and protein transport.For KEGG pathway analysis,these genes are predominantly enriched in protein processing(endoplasmic reticulum),MAPK signaling pathway,and plant hormone signal transduction.Additionally,key genes including 1-aminocyclopropane-1-carboxylate synthase(ACS2)were significantly up-regulated,while mitogen-activated protein kinase(MPK6)and abscisic acid receptor(PYL5)were significantly down-regulated,suggesting that heavy ion beam irradiation regulates hormone synthesis and signal transduction to accelerate leaf abscission.[Conclusion]Heavy ion beam irradiation promotes sugarcane leaf abscission by regulating cellular functions and hormone signaling pathways and activating stress-response mechanisms.These results provide important molecular references for sugarcane genetic improvement and stress response mechanism research.关键词
甘蔗/重离子束/脱叶性突变体/转录组学/候选基因Key words
sugarcane/heavy ion beam/leaf abscission mutant/transcriptomics/candidate genes分类
农业科技引用本文复制引用
李鸣,雷敬超,周会,唐仕云,周利斌,莫丽芳,曲颖,齐浥颖,经艳,方位宽,吴小青,何姗珊,曾媛..重离子束辐照诱导甘蔗易脱叶突变体的转录组学分析及关键候选基因挖掘[J].中国糖料,2026,48(2):1-12,12.基金项目
国家糖料产业技术体系(CARS-17) （CARS-17）
广西科技重大专项(桂科 AA24206005) （桂科 AA24206005）
广西农科院稳定团队项目(桂农科2026YT053)资助. （桂农科2026YT053）
