中国瓜菜2026，Vol.39Issue(4)：1-12,12.DOI:10.16861/j.cnki.zggc.2025.0751
CRISPR/Cas9技术在茄科作物遗传改良中的应用研究进展
Research progress on the application of CRISPR/Cas9 technology in genetic improvement of Solanaceae crops
摘要
Abstract
Gene editing technology provides a crucial tool for crop genetic improvement.As a third-generation gene edit-ing tool,the CRISPR/Cas9 system utilizes guide RNA to specifically recognize target sites and relies on the Cas9 nucle-ase to achieve precise editing:Such as gene knockout,replacement,or insertion—at the target gene locus.With advantag-es including simple design,strong targeting specificity,and high efficiency,the CRISPR/Cas9 gene editing tool has played a vital role in plant functional gene analysis and new variety creation.Solanaceae crops,such as potato,tomato,and pepper,are globally vital economic crops occupying a pivotal position in food security and agricultural development.However,their production is often constrained by factors including pests and diseases,abiotic stress,and quality defects.This paper outlines the fundamental principles of CRISPR/Cas9 technology,analyzes the challenges in editing efficiency and specificity,and reviews its application achievements in the genetic improvement of Solanaceae crops,aiming to pro-vide a scientific reference for precision breeding research and production practices in Solanaceae crops.关键词
茄科作物/CRISPR/Cas9/基因编辑/性状改良Key words
Solanaceae crops/CRISPR/Cas9/Gene editing/Trait improvement分类
农业科技引用本文复制引用
张铭洋,王丹,康国菊,曾祥飞,吕春悦,胡新喜,熊兴耀..CRISPR/Cas9技术在茄科作物遗传改良中的应用研究进展[J].中国瓜菜,2026,39(4):1-12,12.基金项目
国家重点研发计划(2023YFD1201505) （2023YFD1201505）
