中国瓜菜2026，Vol.39Issue(4)：127-133,7.DOI:10.16861/j.cnki.zggc.2025.0559
灵芝胞外粗多糖对常温与高温环境下白灵菇菌丝生长的影响
Effects of Ganoderma lucidum crude exopolysaccharide on the mycelial growth of Pleurotus tuoliensis under normal and high temperature
摘要
Abstract
To shorten the growth cycle of Pleurotus tuoliensis while mitigating the adverse effects of high temperature on its mycelial growth.In this study,Ganoderma lucidum was used as the test material.Crude extracellular polysaccharides of G.lucidum(GLP)were prepared via liquid fermentation and precipitation with ethanol at concentrations of 70％,85％,and 100％,abbreviated as 70％GLP,85％GLP,and 100％GLP,respectively.The effects of these extracellular polysaccha-ride fractions on mycelial growth rate,recovery rate after high-temperature stress,hydrogen peroxide(H2O2)content,malo-ndialdehyde(MDA)content,and intracellular and extracellular enzyme activity of P.tuoliensis were determined.The results showed that the total sugar content among the different fractions of crude G.lucidum polysaccharides followed the order:100％GLP>70％GLP>85％GLP.The results showed that the total sugar content in the different crude GLP frac-tions was in the order of 100％GLP>70％GLP>85％GLP.The 85％GLP fraction at 3.0 mg·mL-1 exhibited the most significant promoting effect on the mycelial growth of P.tuoliensis,with the fastest growth rate reaching 7.13 mm·d-1,and significantly enhanced the intracellular and extracellular enzyme activity of the mycelia.The addition of crude GLP promoted the recovery of P.tuoliensis mycelia after high-temperature stress.Among them,the 85％GLP fraction showed the optimal recovery effect after prolonged stress,with the fastest recovery rate of 5.54 mm·d-1 at 12 h.Meanwhile,it reduced the accumulation of MDA and H2O2 in P.tuoliensis mycelia,improved its stress resistance,and thereby mitigated the negative effects of high temperature.In conclusion,crude extracellular polysaccharides from G.lucidum can promote the mycelial growth of P.tuoliensis,significantly enhance intracellular and extracellular enzyme activities,and alleviate the accumulation of H2O2 and MDA in mycelia under high-temperature stress.The results of this study lay a foundation for the efficient and stable development of the P.tuoliensis industry.关键词
灵芝胞外多糖/白灵菇/菌丝生长/高温胁迫Key words
Ganoderma lucidum exopolysaccharide/Pleurotus tuoliensis/Mycelial growth/High-temperature stress分类
农业科技引用本文复制引用
孙慧娴,张菁,高青云,郭昱秀,孙宁,班立桐,李奕葶..灵芝胞外粗多糖对常温与高温环境下白灵菇菌丝生长的影响[J].中国瓜菜,2026,39(4):127-133,7.基金项目
天津市科技计划项目(23ZYCGSN00870,24ZYCGSN00070,24ZYCGSN00270) （23ZYCGSN00870,24ZYCGSN00070,24ZYCGSN00270）
甘肃省2024年中央引导地方科技发展资金项目(24ZYQH006) （24ZYQH006）
甘肃省科技计划项目(24CXNH019) （24CXNH019）
天津市蔬菜现代农业产业技术体系创新团队(ITTVRS2024016) （ITTVRS2024016）
