中国畜牧兽医2026，Vol.53Issue(5)：2488-2498,11.DOI:10.16431/j.cnki.1671-7236.2026.05.028
复合益生菌剂对多种霉菌毒素攻毒肉鸡的生长性能和毒素残留的影响
Effects of Compound Probiotics on Growth Performance and Toxin Residues in Broilers Challenged with Multiple Mycotoxins
摘要
Abstract
[Objective]This experiment aimed to investigate the effects of a combined probiotic supplement on the growth performance,organ coefficients,serum biochemical indicators,and toxin residues in tissues and feces of broilers exposed to multiple mycotoxins in their feed,namely T-2 toxin(T-2),deoxynivalenol(DON),and ochratoxin A(OTA).[Method]A total of 360 healthy 3-day-old male White-feathered broilers with similar initial body weight(66.65 g±0.50 g)were randomly allotted to 4 groups,each with 6 replicates of 15 birds.The control group(CK)received a basal diet.The mycotoxin challenge group(Challenge),compound probiotic group 1(T1),and compound probiotic group 2(T2)were fed the basal diet supplemented with 100 μg/kg T-2,100 μg/kg DON and 50 μg/kg OTA.Additionally,groups T1 and T2 received probiotic fermentation liquids in their drinking water,T1 received two strains(Brevibacillus laterosporus BLCC1-0170 and Bacillus subtilis BLCC1-1937),while T2 received three strains(Brevibacillus laterosporus BLCC1-0170 and Bacillus subtilis BLCC1-1934,BLCC1-1937),each strain at a final concentration of 1×10⁶ CFU/mL in drinking water.After a 3-day adaptation,the 21-day formal experiment commenced.Feed intake and body weight were recorded to calculate average daily weight gain(ADG)and feed to weight ratio(F/G).On days 7,14,and 21,broilers were fasted,weighed and slaughtered(five broilers were slaughtered each time)to collect organs for index calculation,and serum,liver,kidney,and fecal samples for biochemical and mycotoxin analysis.[Result]① Compared with CK group,the ADG of broilers in challenge group was significantly lower at 7 and 21 days(P<0.05),and the F/G was significantly higher at 21 days(P<0.05);At 21 days,the ADG of broilers in T1 and T2 groups was significantly increased(P<0.05),and the F/G was significantly decreased(P<0.05).② Compared with CK group,the liver index and spleen index of broilers in challenge group were significantly increased at 7 and 14 days(P<0.05),and there was no significant difference in the organ coefficient of broilers in the T1 and T2 groups at 14 days(P>0.05).③ Compared with CK group,the activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in the serum of broilers in challenge group were significantly increased at 7,14 and 21 days(P<0.05),the content of uric acid(UA)in the serum of broilers in challenge group was significantly increased at 14 and 21 days(P<0.05),the contents of serum creatinine(CREA)and albumin(ALB)in the serum of broilers in challenge group were significantly decreased at 21 days(P<0.05),the content of total protein(TP)in the serum of broilers in challenge group was significantly decreased at 14 days(P<0.05),and the content of urea(UREA)in the serum was not significantly affected at 7,14,and 21 days(P>0.05);At 7,14 and 21 days,the activities of AST and ALT in the serum and the contents of ALB and TP in the serum of broilers in T2 group were basically restored to the levels of CK group(P>0.05).④ Broilers in CK group did not detect toxins in the liver,kidney,and feces.Compared with CK group,the contents of DON,OTA and T-2 toxin in the liver,kidney,and feces of broilers in challenge group were significantly increased(P<0.05).Compared with challenge group,the contents of DON,OTA and T-2 toxin in the liver,kidney,and feces of broilers in T2 group(except for OTA content in the kidney at 7 and 14 days)were significantly decreased(P<0.05).[Conclusion]Under the conditions of this experiment,adding T-2 toxin(100 μg/kg),DON(100 μg/kg),and OTA(50 μg/kg)to the feed of broilers can lead to negative impacts on their growth performance and liver and kidney functions.Adding a compound probiotic agent to the drinking water can reduce the accumulation of various mycotoxins in the feces,liver,and kidney of broiler chickens,and alleviate the adverse effects of feed mycotoxin contamination on their growth performance and liver and kidney functions.关键词
霉菌毒素/肉仔鸡/益生菌/毒素残留Key words
mycotoxins/broilers/probiotics/toxin residues分类
农业科技引用本文复制引用
豆屹松,陈威,叶晓红,崔海英,张涵,刘一峰,谷巍..复合益生菌剂对多种霉菌毒素攻毒肉鸡的生长性能和毒素残留的影响[J].中国畜牧兽医,2026,53(5):2488-2498,11.基金项目
国家海外高层次人才引进计划(2023GJJLJRC-112) （2023GJJLJRC-112）
2023山东省重点研发计划(DL2023023005L) （DL2023023005L）
