中国畜牧兽医2026，Vol.53Issue(5)：2609-2618,10.DOI:10.16431/j.cnki.1671-7236.2026.05.038
1株类NADC30猪繁殖与呼吸综合征病毒重组毒株的分离鉴定及致病性分析
Isolation,Identification,and Pathogenicity Analysis of a Recombinant Strain of NADC30-like Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
[Objective]This study aimed to isolate and identify Porcine reproductive and respiratory syndrome virus(PRRSV)from clinically typical cases,analyze the genetic evolution and pathogenicity of epidemic viruses,and provide a reference for PRRSV prevention and control in Shandong region.[Method]The supernatant from homogenized PRRSV-positive lung tissue was inoculated into African green monkey kidney cells(Marc-145)for virus isolation.The isolated strain was identified by indirect immunofluorescence assay(IFA)and transmission electron microscopy(TEM).The whole-genome sequencing and genetic evolution analysis of the isolated strain were conducted.The pathogenicity of the isolated strain was evaluated in 30-day-old piglets.[Result]Following three blind passages,the supernatant from PRRSV-positive lung tissue was used to inoculate Marc-145 cells.After 48 h of infection,Marc-145 cells exhibited obvious cytopathic effects,including shrinkage,rounding,and aggregation.IFA results revealed distinct clustered fluorescence in cells at 24 h post-infection.Transmission electron microscopy observed viral particles 50-60 nm in diameter within cytoplasmic vesicles,consistent with the structural size of PRRSV.These results confirmed the successful isolation of a PRRSV strain,with a viral titer of 105.65 TCID50/mL,which was designated as CH-SDTA-2501.Whole-genome sequencing revealed the full genome of the virus was 15 001 bp in length.Phylogenetic and recombination analysis revealed that CH-SDTA-2501 strain belonged to the NADC30 branch,and it was a recombinant strain derived from NADC30 and JXA1.Piglets was challenged with CH-SDTA-2501(1×105.0 TCID50)via intranasal and intramuscular infection,respectively.After 3 days post-infection,piglets developed respiratory symptoms including coughing and dyspnea.And the virus was detected in serum.Moreover,gross lesions at 14 days post-inoculation showed pulmonary consolidation and interstitial thickening.Histopathological examination further revealed thickened alveolar septa and epithelial cell hyperplasia.[Conclusion]This study successfully isolated a NADC30-like PRRSV strain,which could induce significant respiratory symptoms in piglets.The findings provided novel insights for PRRSV prevention and control.关键词
猪繁殖与呼吸综合征病毒(PRRSV)/NADC30/分离鉴定/遗传进化/致病性Key words
Porcine reproductive and respiratory syndrome virus(PRRSV)/NADC30/isolation and identification/genetic evolution/pathogenicity分类
农业科技引用本文复制引用
梁玥,吴家强,刘宇,李建达,李克欣,于江,曾昊,张玉玉,丁罗刚,刘飞,陈智,孙文博..1株类NADC30猪繁殖与呼吸综合征病毒重组毒株的分离鉴定及致病性分析[J].中国畜牧兽医,2026,53(5):2609-2618,10.基金项目
国家自然科学基金(32402881) （32402881）
山东省现代农业产业技术体系(SDAIT-08) （SDAIT-08）
济南市新高校20条(202333065) （202333065）
山东省农业科学院农业科技创新工程(CXGC2025F09、CXGC2025C10) （CXGC2025F09、CXGC2025C10）
泰山学者工程资助项目 （）
