中国烟草学报2026，Vol.32Issue(2)：72-83,12.DOI:10.16472/j.chinatobacco.2025.T0389
烟草毛状根诱导过程中组织的差异表达基因分析
Differential gene expression analysis in tissues during tobacco hairy root induction
摘要
Abstract
[Background]Infection of plants by Agrobacterium rhizogenes can induce hairy root formation;however,the dynamic and systematic gene regulatory network underlying this process remains unclear.This study aims to elucidate the molecular mechanisms underlying hairy root formation.[Methods]This study aims to elucidate the temporal changes in gene expression during the infection of tobacco explants by Agrobacterium rhizogenes using RNA-seq combined with weighted gene co-expression network analysis(WGCNA)and transcription factor analysis.[Results]With prolonged infection time,the number of differentially expressed genes(DEGs)gradually increased.These DEGs were significantly enriched in processes such as oxidoreductase activity and metabolic reprogramming,and a total of 2,497 DEGs were shared among all comparison groups.WGCNA identified one module significantly positively correlated and one module significantly negatively correlated with Agrobacterium rhizogenes infection,each containing two hub genes.Auxin response factors(ARFs)were the most significantly enriched transcription factor family and showed strong evolutionary conservation.Integrated analysis identified a regulatory network for hairy root formation centered on a lateral organ boundary domain protein gene,including TCP family transcription factors,ethylene response transcription factors,and key ARF genes.[Conclusion]This study provides a theoretical basis and identifies key gene targets for both fundamental research in hairy root biology and its biotechnological applications.关键词
烟草毛状根/转录组学/差异表达基因/加权基因共表达分析(WGCNA)/生长素响应因子(ARF)Key words
tobacco hairy roots/transcriptomics/differentially expressed genes/weighted gene co-expression network analysis(WGCNA)/auxin response factor(ARF)引用本文复制引用
闫浩宇,林娟,张勇刚,任志广,朱智文,齐志韬,陶丰,唐贵良,石超男,吴晓宗..烟草毛状根诱导过程中组织的差异表达基因分析[J].中国烟草学报,2026,32(2):72-83,12.基金项目
河南省科技攻关项目"调控类黄酮生物合成的关键技术开发研究"(项目编号:252102110270) （项目编号:252102110270）
