中国烟草学报2026，Vol.32Issue(2)：127-134,8.DOI:10.16472/j.chinatobacco.2025.T0321
烟草赤星病与野火病复合侵染烟叶叶际微生物群落结构与多样性
Microbial community structure and diversity in the phyllosphere of tobacco leaves co-infected with brown spot and wildfire diseases
摘要
Abstract
[Background]This study aims to elucidate the characteristics of microbial community structure and diversity in the phyllosphere of tobacco leaves co-infected with brown spot and wildfire diseases.[Methods]Asymptomatic tissues and diseased tissues were collected from typical infected tobacco leaves at disease severity grades 1,3,5,and 7.Illumina high-throughput sequencing was employed to analyze the fungal and bacterial community structures associated with asymptomatic and diseased leaf tissues.[Results]Significant differences in fungal and bacterial community diversity were observed between asymptomatic and diseased tobacco leaf tissues across different disease severity levels.Fungal diversity was higher in asymptomatic leaf tissues,whereas diseased tissues exhibited reduced fungal diversity.At the genus level,the dominant fungal genera in diseased tissues included Alternaria and Filobasidium.The relative abundance of Alternaria in leaves with disease grades 1,3,5,and 7 was 53.37％,61.72％,63.12％,and 82.67％,respectively.In contrast,asymptomatic leaf tissues were mainly dominated by Filobasidium and Cladosporium,with relative abundances ranging from 6.41％to 39.07％and 5.50％to 17.04％,respectively.The dominant bacterial genera in diseased leaf tissues were Pantoea and Pseudomonas.The relative abundance of Pseudomonas in leaves with disease grades 1,3,5,and 7 was 18.01％,55.15％,38.71％,and 30.90％,respectively.Asymptomatic leaf tissues were primarily dominated by Pantoea,with relative abundances ranging from 16.69％to 56.43％.Furthermore,the relative abundances of Pseudomonas,Xerobacter,and Xanthomonas were significantly higher in diseased leaf tissues than in asymptomatic tissues.[Conclusion]This study reveals distinct phyllosphere microbial characteristics associated with the co-infection of brown spot and wildfire diseases in tobacco leaves,providing a reference for understanding the mechanisms underlying the combined occurrence of these two diseases.关键词
烟草赤星病/烟草野火病/Illumina高通量测序/群落结构与多样性Key words
tobacco brown spot/tobacco wildfire/Illumina high-throughput sequencing/community structure and diversity引用本文复制引用
秦子朕,史腾飞,宋媛,胡静荣,史彩华,蔡刘体,陆宁,汪汉成..烟草赤星病与野火病复合侵染烟叶叶际微生物群落结构与多样性[J].中国烟草学报,2026,32(2):127-134,8.基金项目
中国烟草总公司科技项目(110202101048(LS-08)) （110202101048(LS-08）
中国烟草总公司贵州省公司科技项目(2024XM06),贵州省"百层次"创新型人才(黔科合平台人才-GCC[2022]028-2、黔科合平台人才-GCC[2023]108) （2024XM06）
贵州省科技创新人才团队项目(黔科合平台人才-CXTD[2023]021) （黔科合平台人才-CXTD[2023]021）
