中国药房2026，Vol.37Issue(8)：998-1002,5.DOI:10.6039/j.issn.1001-0408.2026.08.05
刺五加苷B调控IKKβ/NF-κB信号通路对帕金森病模型小鼠的神经保护作用及机制
Neuroprotective effect and mechanism of eleutheroside B on Parkinson's disease model mice by regulating the IKKβ/NF-κB signaling pathway
摘要
Abstract
OBJECTIVE To investigate the neuroprotective effect and mechanism of eleutheroside B (ELB) on Parkinson's disease (PD) model mice by regulating the IκB kinase β (IKKβ)/nuclear factor-κB (NF-κB) signaling pathway.METHODS Fifty mice were randomly divided into normal control group,model group,positive control group (selegiline hydrochloride,10 mg/kg),and ELB low-dose and high-dose groups(80,160 mg/kg),with 10 mice in each group.Each group was given relevant medicine or normal saline intragastrically for 14 consecutive days.Starting from the 10th day of administration,the model group and all administration groups were intraperitoneally injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)30 mg/kg,for five consecutive days to establish the chronic PD model.After the last administration for 24 h,six mice were randomly selected from each group to test their behavioral abilities;detect the levels of interleukin-1β(IL-1β),IL-10,tumor necrosis factor-α(TNF-α)in brain tissue and their mRNA expressions were measured,and positive expression of tyrosine hydroxylase(TH),protein expressions of TH,α-synuclein(α-syn),ionized calcium-binding adaptor molecule 1(Iba-1),as well as phosphorylation levels of IKKβ and NF-κB p65 proteins in the brain tissue were detected.The ultrastructure of neurons in substantia nigra was observed.RESULTS Compared with the model group,rotarod endurance time and climbing score of each administration group(except for the ELB low-dose group)were increased significantly(P<0.05),while the levels and mRNA expressions of IL-1β,TNF-α,α-syn,and Iba-1,as well as phosphorylation levels of IKKβ and NF-κB p65 proteins in brain tissue were decreased significantly(except for TNF-α in the ELB low-dose group).Conversely,the level and mRNA expression of IL-10(except for the ELB low-dose group),TH positive expression and protein expressions were significantly increased(P<0.05).Typical neurodegenerative pathological changes,such as neuronal karyopyknosis,mitochondrial swelling and vacuolization,and endoplasmic reticulum dilation,all showed varying degrees of improvement.CONCLUSIONS ELB may exert neuroprotective effects by inhibiting the activation of the IKKβ/NF-κB signaling pathway,alleviating inflammatory responses,reducing abnormal α-syn aggregation and neuronal loss,and further improving motor dysfunction in PD mice.关键词
刺五加苷B/帕金森病/行为能力/神经炎症/神经保护/IKKβ/NF-κB信号通路Key words
eleutheroside B/Parkinson's disease/behavioral ability/neuroinflammation/neuroprotection/IKKβ/NF-κB signaling pathway分类
医药卫生引用本文复制引用
王晓丽,李晓明,荣华,潘思文,于春磊,徐天娇,孙宇,丛欢,庞雨,陈刚..刺五加苷B调控IKKβ/NF-κB信号通路对帕金森病模型小鼠的神经保护作用及机制[J].中国药房,2026,37(8):998-1002,5.基金项目
国家自然科学基金项目(No.82505000) （No.82505000）
齐齐哈尔市科技计划联合引导项目(No.LSFGG-2025136) （No.LSFGG-2025136）
齐齐哈尔医学科学院临床科研基金项目(No.QMSI2024L-01,No.QMSI2024L-09) （No.QMSI2024L-01,No.QMSI2024L-09）
齐齐哈尔医学院重点学科建设项目(No.QYZDXK-007) （No.QYZDXK-007）
齐齐哈尔医学科学院面上项目(No.QMSI2024M-09) （No.QMSI2024M-09）
