中国医科大学学报2026，Vol.55Issue(4)：295-300,6.DOI:10.12007/j.issn.0258-4646.2026.04.002
紫草素调控AMPK/mTOR信号通路对人食管癌细胞增殖、凋亡、迁移、侵袭和顺铂耐药的影响
Impact of shikonin on proliferation,apoptosis,migration,invasion,and cisplatin resistance of human esophageal cancer cells through AMPK/mTOR signaling pathway regulation
摘要
Abstract
Objective To investigate the impact of shikonin on proliferation,apoptosis,migration,invasion,and cisplatin resistance of human esophageal cancer cells through the regulation of the adenosine 5'-monophosphate-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)signaling pathway.Methods KYSE150 cells in logarithmic growth phase were assigned to the control,low-shikonin(0.5 mol/L),high-shikonin(2.0 mol/L),compound C(10 μmol/L),and high-shikonin+compound C groups.After 24 h of incubation,the cell proliferation rate and drug resistance sensitivity were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide(MTT)assay;cell apoptosis,migration,and invasion were examined using flow cytometry and the Transwell assay;and the expression levels of autophagy-related gene(P62 and LC3)mRNAs were determined using real-time quantitative PCR.The expression of multidrug resistance-related protein 1(MRP1),PIN2/TERF1-interacting telomerase inhibitor 1(PINX1),proliferating cell nuclear antigen(PCNA),Bcl-2 associated X protein(Bcl-2)associated X protein,phosphorylated(p)-AMPK/AMPK,and p-mTOR/mTOR was analyzed using Western blotting.Results Compared with the control group,the low-and high-shikonin groups showed decreased absorbance(OD490),half-maximal inhibitory concentration(IC50),invasion and migration numbers,P62 mRNA expression,p-mTOR/mTOR,MRP1,and PCNA expressions(P<0.05),whereas the apoptosis rate as well as LC3 mRNA,p-AMPK/AMPK,PINX1,and Bax expressions increased.Compound C weakened the inhibitory effect of high levels of shikonin on the malignant behavior of KYSE150 cells.Conclusion Shikonin inhibits the proliferation,migration,and invasion of human esophageal cancer cells,promotes apoptosis,and enhances cisplatin sensitivity by upregulating the AMPK/mTOR signaling pathway.关键词
紫草素/AMPK/mTOR信号通路/食管癌/增殖/迁移与侵袭/顺铂/凋亡Key words
shikonin/AMPK/mTOR signaling pathway/esophageal cancer/proliferation/migration and invasion/cisplatin/apoptosis分类
医药卫生引用本文复制引用
翟明慧,袁殿宝,吴文娟..紫草素调控AMPK/mTOR信号通路对人食管癌细胞增殖、凋亡、迁移、侵袭和顺铂耐药的影响[J].中国医科大学学报,2026,55(4):295-300,6.基金项目
河北省医学科学研究课题计划(20210617) （20210617）
