中国医科大学学报2026，Vol.55Issue(4)：323-328,6.DOI:10.12007/j.issn.0258-4646.2026.04.006
经颅直流电刺激通过抑制MCP-1/CCR2信号通路减轻膝骨关节炎大鼠疼痛症状
Transcranial direct current stimulation alleviates pain in rats with knee osteoarthritis by inhibiting MCP-1/CCR2 signaling pathway
摘要
Abstract
Objective To explore the mechanism by which transcranial direct current stimulation(tDCS)alleviates pain in rats with knee osteoarthritis(KOA)via the monocyte chemoattractant protein 1(MCP-1)/C-C motif chemokine receptor 2(CCR2)signaling pathway.Methods Male SD rats were randomly divided into sham,model,tDCS,and tDCS+MCP-1 groups,with 10 rats in each group.Thermal withdrawal latency(TWL)and mechanical withdrawal threshold(MWT)were measured.The expression and localization of the pain mediator nerve growth factor(NGF)protein in knee joint tissue and ionized calcium-binding adapter molecule 1(Iba1)protein in dorsal root ganglia were detected by immunohistochemistry.The expression of interleukin-6(IL-6)and interleukin-1β(IL-1β)proteins in knee joint tissue,pain-related proteins tropomyosin receptor kinase A(TrkA),growth-associated protein 43(GAP43),and calcitonin gene-related peptide(CGRP)in dorsal root ganglia,and MCP-1 and CCR2 proteins in knee joint tissue and spinal cord was detected by Western blotting.Results Compared with the sham group,TWL and MWT decreased(P<0.05),and the levels of NGF,IL-6,IL-1β,Iba1,TrkA,GAP43,CGRP,MCP-1,and CCR2 increased in the model group(P<0.05).Compared with the model group,these indicators improved in the tDCS group(P<0.05).Compared with the tDCS group,these indicators were partly reversed in the tDCS+MCP-1 group(P<0.05).Conclusion tDCS alleviates pain symptoms in KOA rats by inhibiting pro-inflammatory factors and pain-related proteins,and the mecha-nism may be related to the inhibition of the MCP-1/CCR2 signaling pathway.关键词
经颅直流电刺激/单核细胞趋化蛋白1/趋化因子受体2/膝骨关节炎/疼痛Key words
transcranial direct current stimulation/monocyte chemoattractant protein 1/C-C motif chemokine receptor 2/knee osteo-arthritis/pain分类
医药卫生引用本文复制引用
郭仁艺,符英杰,林丁,王运成..经颅直流电刺激通过抑制MCP-1/CCR2信号通路减轻膝骨关节炎大鼠疼痛症状[J].中国医科大学学报,2026,55(4):323-328,6.基金项目
海南省卫生健康行业科研项目(22A200044) （22A200044）
