中国药理学与毒理学杂志2026，Vol.40Issue(2)：104-114,11.DOI:10.3867/j.issn.1000-3002.2026.08724
归脾汤对慢性束缚应激小鼠焦虑样行为的改善作用及机制
Effect of Guipi decoction on anxiety-like behaviors of chronic restraint stress mice and its mechanism
徐晴 1胡素敏 2范琼尹 3郭昱含 3高源锴 2徐旻灏 2王艳 2张文彦 2叶一帆 2张有志3
作者信息
- 1. 北京中医药大学中医学院,北京 100029||军事医学研究院,北京 100850||国家安全特需药品全国重点实验室,北京 100039
- 2. 北京中医药大学中医学院,北京 100029
- 3. 军事医学研究院,北京 100850||国家安全特需药品全国重点实验室,北京 100039
- 折叠
摘要
Abstract
OBJECTIVE To observe the ameliorative effects of Guipi decoction(GP)on anxiety-like behaviors in a chronic restraint stress(CRS)mouse model and to explore its underlying mecha-nisms.METHODS The major chemical components of GP aqueous extract were identified by-ultra-per-formance liquid chromatography coupled with quadrupole-exactive Orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap MS).Male C57BL/6J mice were randomly divided into control group,model group,model+buspirone 5mg·kg-1 group,and model+GP 1.09,2.18 and 4.36g·kg-1 groups,with continuous intragastric administration for 16 days.Except for the control group,mice in other groups were subjected to the CRS procedure 1 hour after daily administration for 14 consecutive days.Anxiety-like behaviors were assessed using the open field test,elevated plus maze test(day15),and light-dark box test(day16).Pathological changes in hippocampal neurons were observed by HE staining and Golgistaining.The positive expressions of silent information regulator 1(SIRT1)and brain-derived neurotrophic factor(BDNF)were detected by immunohistochemistry.Western blotting was employed to measurethe expression levels of postsynaptic density protein 95(PSD95),SIRT1,BDNF,and tropomyosin receptor kinase B(TrkB),as well as the phosphorylation levels of extracellular signal-regulated kinase(ERK)and cAMP response element-binding protein(CREB).RESULTS Compared with control group,anxiety-like behaviors,neuronal damage,and synaptic plasticity-related proteins in model group were signifi-cantly reduced.Compared with model group,these behavioral and pathological abnormalities in groups treated with model+GP(2.18 and 4.36 g·kg-1)were significantly ameliorated.Furthermore,the expres-sion of PSD95,SIRT1,BDNF,TrkB,and the phosphorylation levels of ERK and CREB in model+GP2.18 g·kg-1 were significantly up-regulated.CONCLUSION Anxiety-like behaviors in CRS model mice were significantly ameliorated by GP,and its mechanism maybe associated with the improvement of synaptic plasticity via the SIRT1/CREB/BDNF signaling pathway.关键词
焦虑症/慢性束缚应激/归脾汤/神经保护/突触可塑性/沉默信息调节因子1/行为学Key words
anxiety disorder/chronic restraint stress/Guipi decoction/neuroprotection/synaptic plasticity/silent information regulator 1/behavior分类
医药卫生引用本文复制引用
徐晴,胡素敏,范琼尹,郭昱含,高源锴,徐旻灏,王艳,张文彦,叶一帆,张有志..归脾汤对慢性束缚应激小鼠焦虑样行为的改善作用及机制[J].中国药理学与毒理学杂志,2026,40(2):104-114,11.
