中国药业2026，Vol.35Issue(8)：38-43,6.DOI:10.3969/j.issn.1006-4931.2026.08.008
靶向IgE重链Fc段潜在拮抗药物筛选及拮抗效果分析
Screening of Potential Antagonistic Drugs Targeting IgE Heavy Chain Fc Fragment and Analysis of the Antagonistic Effects
摘要
Abstract
Objective To screen potential antagonistic drugs targeting immunoglobulin E(IgE)heavy chain Fc fragment,and evaluate their antagonistic effects.Methods Surface plasmon resonance(SPR)technology was used to perform high-throughput screening for drugs from the U.S.Food and Drug Administration approved drug library that bind to the recombinant human IgE Fc fragment(rhIgE-Fc,500,250,125,62.5,and 31.3 nmol/L).Molecular docking models of candidate drugs with the rhIgE protein were simulated,and the antagonistic effects of the candidate drugs against rhIgE were evaluated by indirect competitive enzyme-linked immunosorbent assay.Results Three drugs with strong binding affinity to the rhIgE-Fc fragment protein were identified:potassium andrographolide succinate,pinaverium bromide,and lipoic acid.The binding affinities of these three drugs were positively correlated with the concentration of the rhIgE-Fc fragment protein.Molecular docking results showed that all three candidate drugs effectively occupied the Fc fragment binding pocket of the rhIgE fragment protein,with molecular docking binding energies of-6.0,-5.3,and-4.4 kcal/mol,respectively.Compared with the currently clinically used IgE monoclonal antibody omalizumab(which exhibited a 31.57% antagonistic effect against rhIgE at 120 µg/mL),the mass concentrations required for potassium andrographolide succinate,pinaverium bromide,and lipoic acid to achieve an equivalent antagonistic effect were 86.04,60.86,and 167.23 ng/mL,respectively.Conclusion Potassium andrographolide succinate,pinaverium bromide,and lipoic acid can be used as potential IgE antagonist drugs for the treatment of acute allergic and chronic inflammatory allergic diseases.关键词
IgE Fc/高通量筛选/表面等离子体共振/拮抗药物Key words
IgE Fc/high-throughput screening/surface plasmon resonance/antagonistic drug分类
医药卫生引用本文复制引用
唐蕾,王海,张颖宜,张转铃,黄艳..靶向IgE重链Fc段潜在拮抗药物筛选及拮抗效果分析[J].中国药业,2026,35(8):38-43,6.基金项目
广东省基础与应用基础研究基金项目[2023A1515140139]. （）
