中国药业2026，Vol.35Issue(8)：75-80,6.DOI:10.3969/j.issn.1006-4931.2026.08.014
冬凌草饮片中外源性有害物质残留检测
Residues of Exogenous Harmful Substances in Rabdosiae Rubescentis Herba Pieces
摘要
Abstract
Objective To establish a method for the determination of exogenous harmful substances(including heavy metals and harmful elements,as well as plant growth regulators)in Rabdosiae Rubescentis Herba pieces.Methods The samples were pretreated by microwave digestion.Inductively coupled plasma mass spectrometry(ICP-MS)was used to determine the contents of five heavy metals and harmful elements,including lead(Pb),cadmium(Cd),arsenic(As),mercury(Hg)and copper(Cu)in the samples.Ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to determine the residues of 24 plant growth regulators,including atrazine,butralin,chlormequat chloride,fruitone CPA,chlorpropham,guayule,2-diethylaminoethyl hexanoate,1,3-diphenylurea,ethychlozate,flumetralin,flurprimidol,kinetin,inabenfide,mepiquat,enadenine,naphthylacetamide,paclobutrazol,pyripropanol,trans-zeatin,triapenthenol,uniconazole,6-benzylaminopurine,forchlorfenuron and thidiazuron.Results All heavy metals and harmful elements,as well as plant growth regulators showed good linear relationships with the instrument response value of analyte/internal standard element or peak area in the corresponding concentration ranges(r>0.990,n=6).The limits of detection were 0.000 2-0.001 0 mg/kg and 0.01-0.10 mg/kg,respectively;the limits of quantitation were 0.000 5-0.003 5 mg/kg and 0.03-0.30 mg/kg,respectively.The RSDs of precision and stability tests were all lower than 5.0%.The average recoveries were 90.18%-105.12%(with RSDs of 0.59%-2.22%)and 75.31%-90.82%(with RSDs of 0.31%-4.61%),respectively(n=6).Heavy metals were detected in all 27 batches of samples,among which four batches exceeded the limit of Pb,one batch exceeded the limit of Hg and one batch exceeded the limit of Cd.Pyripropanoll residues were detected in five batches of samples,the contents range was 0.012-0.096 mg/kg,all of which were lower than the limit of quantitation(0.15 mg/kg).Conclusion The established method is simple and accurate,and can be used for the control of exogenous harmful substances in Rabdosiae Rubescentis Herba pieces.关键词
冬凌草饮片/电感耦合等离子体质谱法/超高效液相色谱串联质谱法/重金属/有害元素/植物生长调节剂Key words
Rabdosiae Rubescentis Herba pieces/inductively coupled plasma mass spectrometry/ultra-performance liquid chromatography-tandem mass spectrometry/heavy metal/harmful element/plant growth regulator分类
医药卫生引用本文复制引用
杨秋红,李珊,黄霞,李海燕,刘亚楠,王海波..冬凌草饮片中外源性有害物质残留检测[J].中国药业,2026,35(8):75-80,6.基金项目
国家药品监督管理局药品监管科学体系建设重点项目[RS2024Z006] （）
国家药品监督管理局中药材及饮片质量控制重点实验室项目[ZZ202303] （）
河南省市场监督管理局科技计划项目[HNSCJGK202424]. （）
