中国中药杂志2026，Vol.51Issue(8)：2295-2302,8.DOI:10.19540/j.cnki.cjcmm.20251119.801
淫羊藿与女贞子配伍对糖皮质激素合成受限哮喘大鼠模型的作用机制
Therapeutic mechanism of combination of Epimedii Folium and Ligustri Lucidi Fructus for glucocorticoid synthesis-restricted asthma in rats
摘要
Abstract
This study aims to elucidate the therapeutic effects and mechanisms of the combination of Epimedii Folium and Ligustri Lucidi Fructus on glucocorticoid(GC)synthesis-restricted asthma in rats.The classic asthma model induced by ovalbumin(OVA)sensitization and challenge,a complex asthma animal model with specific suppression of hypothalamic-pituitary-adrenal(HPA)axis at the synthesis level of endogenous GC by intraperitoneal administration of metyrapone(Met),was established.This model was designed to simulate the pathological condition of GC resistance or insufficient synthesis encountered in clinical practice.Male SD rats were randomly assigned into six groups:normal,Met,asthma+Met,Epimedii Folium,Ligustri Lucidi Fructus,and Epimedii Folium+Ligustri Lucidi Fructus,followed by a one-week drug treatment via gavage.Serum levels of corticotropin-releasing hormone(CRH),adrenocorticotropic hormone(ACTH),corticosterone(CORT),and cortisol(COR)were assessed.Targeted metabolomics was adopted to detect endogenous GC metabolism levels in the urine.Immunohistochemical staining was employed to assess the expression of hydroxysteroid dehydrogenase(HSD)11B1,HSD11B2,cytochrome P450 family 11 subfamily B member 1(CYP11B1),and steroidogenic factor 1(SF1)in the lung and adrenal gland.Western blot was employed to quantify the expression of related proteins in the liver tissue.The results showed that Epimedii Folium and/or Ligustri Lucidi Fructus elevated the serum concentrations of CRH,ACTH,CORT,and COR in model rats,partially reversed abnormalities in the urinary GC metabolic profile,and upregulated the expression of key synthetic and metabolic enzymes(HSD11B1,HSD11B2,CYP11B1,and SF1)in the lung,liver,and adrenal gland.The combination of Epimedii Folium and Ligustri Lucidi Fructus exerts multi-faceted regulatory effects on both the HPA axis and GC synthesis/metabolism in the peripheral tissue,synergistically ameliorating endogenous GC dysregulation in synthesis-restricted asthma.These findings suggest the potential therapeutic value of this combination against GC resistance and related disorders.关键词
淫羊藿-女贞子/哮喘/美替拉酮/内源性糖皮质激素/HPA 轴Key words
Epimedii Folium-Ligustri Lucidi Fructus/asthma/metyrapone/endogenous glucocorticoids/HPA axis引用本文复制引用
马再娜,刘仁慧,谢永豪,马紫童,李玉曼,龙玉婷,唐秀凤..淫羊藿与女贞子配伍对糖皮质激素合成受限哮喘大鼠模型的作用机制[J].中国中药杂志,2026,51(8):2295-2302,8.基金项目
国家自然科学基金青年科学基金项目(82204880) （82204880）
山东省自然科学基金青年科学基金项目(ZR2022QH049) （ZR2022QH049）
山东省中医药科技面上项目(M20243901) （M20243901）
新疆医科大学校级自然科学青年研究项目(2026XYZR46) （2026XYZR46）
