浙江大学学报（农业与生命科学版）2026，Vol.52Issue(2)：270-280,11.DOI:10.3785/j.issn.1008-9209.2025.04.253
姜荷花粉色苞片花青素形成的关键基因及呈色机制分析
Analysis of key genes involved in anthocyanidin formation in pink bracts and coloration mechanisms in Curcuma alismatifolia
摘要
Abstract
Curcuma alismatifolia is a perennial rhizomatous herbaceous flower belonging to the genus Curcuma,with flower color improvement serving as a key breeding objective.In this study,the anthocyanidin components in the pink sterile bracts of C.alismatifolia'Chiang Mai Pink'were analyzed using high performance liquid chromatography.Four anthocyanidins were identified:malvidin,delphinidin,cyanidin and pelargonidin,with malvidin exhibiting the highest proportion.The contents of cyanidin and malvidin increased significantly with the opening process of the flower,while the content of pelargonidin reached its peak when the flower was semi-open.Based on full-length transcriptome sequencing data from the PacBio platform,full-length sequences of nine key genes(PAL1,C4H1,4CL1,CHS1,F3H1,F3´H1,F3´5´H1,ANS1,and 3GT1)involved in the anthocyanidin biosynthesis pathway were obtained.Tissue-specific expression verification was performed for F3´5´H1,F3H1,and ANS1,revealing that their expression levels in sterile bracts and single flowers were significantly higher than those in fertile bracts and transitional bracts.Correlation analysis showed that the contents of cyanidin,delphinidin,and malvidin were positively correlated to varying degrees with the expression levels of the aforementioned nine genes and DFR(GenBank accession number GU126388.1),whereas the changes in pelargonidin content exhibited inconsistent correlations.Based on the comprehensive analysis of anthocyanidin composition,dynamic changes,and spatiotemporal characteristics of gene expression,it is concluded that the anthocyanidin substances responsible for the pink coloration of sterile bracts in'Chiang Mai Pink'are cyanidin and pelargonidin from the red series,along with a certain amount of delphinidin from the blue-purple series and its derivative malvidin;among them,malvidin plays an important role in the coloration of the purple petals of the florets.This study provides a theoretical basis for elucidating the molecular mechanisms underlying coloration in C.alismatifolia and for the targeted improvement of flower color.关键词
姜荷花/苞片/花青素/花色形成/关键基因Key words
Curcuma alismatifolia/bract/anthocyanidin/flower color formation/key genes分类
农业科技引用本文复制引用
刘建新,蔡淑钰,吴丽元,罗洁,宋江平,胡绍泉..姜荷花粉色苞片花青素形成的关键基因及呈色机制分析[J].浙江大学学报（农业与生命科学版）,2026,52(2):270-280,11.基金项目
浙江省重点研发计划项目(2019C02025) （2019C02025）
绍兴理工学院院级重点学科项目(9999520012). （9999520012）
