浙江大学学报（农业与生命科学版）2026，Vol.52Issue(2)：281-293,13.DOI:10.3785/j.issn.1008-9209.2025.03.261
斜纹夜蛾雄蛾对种间性信息素的嗅觉反应
Olfactory responses of male Spodoptera litura to interspecific sex pheromones
摘要
Abstract
To elucidate the olfactory mechanism underlying interspecific chemical communication in Spodoptera litura,this study used S.litura as the subject of investigation.Through field trapping experiments,we determined the modulatory effects of interspecific pheromones on the trapping of male moths by sexual pheromones.Using two-electrode voltage clamp techniques,we characterized the responses of pheromone receptors of S.litura to interspecific pheromones from both S.litura and other pest species in its habitat.Field experiments revealed that interspecific pheromones,specifically Z7-12:Ac and Z9-14:OH,strongly reduced the trapping efficacy of S.litura males,and the effects of intraspecific trace components varied regionally.Electrophysiological analysis revealed that SlituOR6 exhibited a strong current response to E11-14:Ac and a weaker current response to 16:Ac.SlituOR16,however,displayed the highest sensitivity to the S.exigua sex pheromone component Z9-14:OH,a moderate sensitivity to the Agrotis ipsilon sex pheromone component Z7-12:Ac,and a weak response to the S.litura sex pheromone trace component E11-14:Ac.These findings suggest that SlituOR16 plays a dual role in detecting both intraspecific and interspecific pheromone signals.Fluorescence in situ hybridization(FISH)further localized SlituOR16 to receptor neurons at the base of the antennae,suggesting that SlituOR16 may be involved in the recognition of sex pheromones.Collectively,this study provides evidence of SlituOR6 and SlituOR16 specific responses to interspecific sex pheromone signals,elucidating the molecular basis of interspecific chemical communication in S.litura.Additionally,it highlights the interplay between receptor specificity and environmental background odors in modulating these interactions and offers a theoretical foundation for pheromone-based monitoring and control.关键词
斜纹夜蛾/种间化学通信/种间性信息素/性信息素受体/双电极电压钳/性信息素诱捕Key words
Spodoptera litura/interspecific chemical communication/interspecific sex pheromone/sex pheromone receptor/two-electrode voltage clamp/sex pheromone trapping分类
农业科技引用本文复制引用
张玥滢,李佳颍,肖艳松,曾维爱,滕凯,陈梦丹,刘天波,伍绍龙,杜永均..斜纹夜蛾雄蛾对种间性信息素的嗅觉反应[J].浙江大学学报（农业与生命科学版）,2026,52(2):281-293,13.基金项目
中国烟草总公司重大科技项目[110202201027(LS-11)] （LS-11）
湖南省烟草公司重大科技项目(HN2023KJ21). （HN2023KJ21）
