浙江大学学报（农业与生命科学版）2026，Vol.52Issue(2)：327-342,16.DOI:10.3785/j.issn.1008-9209.2025.04.251
重金属镉胁迫对南美白对虾肝胰腺组织中蛋白质组表达的影响
Effects of heavy metal Cd stress on proteome expression in the hepato-pancreas of Litopenaeus vannamei
摘要
Abstract
This study employed tandem mass tag(TMT)-labeled proteomics technology to investigate the changes in protein expression in the hepatopancreas of Litopenaeus vannamei under 0.2 mg/L Cd2+stress for 6 d,aiming to elucidate the Cd accumulation and tolerance mechanisms in typical marine crustaceans.The results revealed a total of 369 differential proteins in the stress group compared with the control group,including 214 upregulated and 155 downregulated proteins.The gene ontology(GO)analysis indicated that these differential proteins were primarily involved in biological processes such as cellular process and metabolic process,cellular components including cells and protein-binding complexes,and molecular functions dominated by catalytic activity and binding.The eukaryotic orthologous groups(KOG)annotation showed significant enrichment in functions such as post-translational modification and energy metabolism.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis demonstrated that upregulated proteins were associated with the protein processing in endoplasmic reticulum pathway,while downregulated proteins were concentrated in ribosome-related pathways,as well as arginine and proline metabolism pathways.Under the Cd stress,key proteins such as zinc finger proteins,metallothionein-like,calnexin,calreticulin,heat shock proteins(HSPs)family(HSP60,HSP70,and HSP90),and antioxidant enzymes like glutathione peroxidase(GPx)were significantly upregulated.These proteins may collectively form a molecular response network in Litopenaeus vannamei to counteract Cd2+stress by regulating Cd2+binding and efflux,repairing misfolded proteins,alleviating endoplasmic reticulum stress,and scavenging reactive oxygen species.关键词
南美白对虾/镉/蛋白质组/富集机制/抗氧化酶Key words
Litopenaeus vannamei/Cd/proteome/enrichment mechanism/antioxidant enzyme分类
生物科学引用本文复制引用
梅光明,顾捷,何鹏飞,许丹,陈思,张小军,杨文鸽..重金属镉胁迫对南美白对虾肝胰腺组织中蛋白质组表达的影响[J].浙江大学学报（农业与生命科学版）,2026,52(2):327-342,16.基金项目
浙江省科技厅科研院所专项(HYS-CZ-202610) （HYS-CZ-202610）
浙江省基础公益研究计划项目(LTGN23C200003). （LTGN23C200003）
