浙江农业科学2026，Vol.67Issue(4)：878-883,6.DOI:10.16178/j.issn.0528-9017.20250792
VIGS介导的NtHD20基因沉默促进烟草腺毛发生
VIGS mediated NtHD20 gene silencing promotes tobacco glandular trichome pilogenesis
摘要
Abstract
This study used the wild-type strain 8306(WT),homozygous edited strain(KD),and overexpressed strain(OE)as materials to investigate the effects of virus-induced gene silencing(VIGS)targeting the NtHD20 gene on the glandular trichome phenotype,glandular trichome density,content of glandular trichome secretions,and expression levels of key biosynthesis genes of glandular trichome secretions in all tobacco lines.The results showed that silencing the NtHD20 gene resulted in an extremely significant(p＜0.01)increase in leaf glandular trichome density,a significant(p＜0.05)increase in content of glandular trichome secretions,and expression levels of key biosynthesis genes of glandular trichome secretions in all tobacco strains.Silencing NtHD20 with VIGS technology can promote the development of tobacco glandular trichome and increase the content of cembratrieneols substances.It is speculated that the NtHD20 gene may have a negative regulatory effect on the development of the tobacco glandular trichome,providing a new direction for improving the tobacco glandular trichome.关键词
烟草腺毛/腺毛分泌物/病毒诱导的基因沉默Key words
tobacco glandular trichome/glandular trichome secretion/virus-induced gene silencing分类
农业科技引用本文复制引用
徐鸣锴,高鑫,王东,鲁宇童,李红涛,陈孟起,段卫东,申洪涛,徐世晓..VIGS介导的NtHD20基因沉默促进烟草腺毛发生[J].浙江农业科学,2026,67(4):878-883,6.基金项目
中国烟草总公司河南省公司创新项目(2024410000240027) （2024410000240027）
河南中烟工业有限责任公司科技项目(2024410000340192) （2024410000340192）
安徽省烟草专卖局(公司)科技项目(2024341800040184) （公司）
贵州省烟草公司安顺市公司重点研发项目(2024520400240023) （2024520400240023）
