杂交水稻2026，Vol.41Issue(2)：11-20,10.DOI:10.16267/j.cnki.1005-3956.20250214.025
水稻耐热基因TT2的特异性鉴定标记开发
Development of Markers for Specific Identification of the Heat-tolerant Gene TT2 in Rice
摘要
Abstract
This study developed high-efficiency molecular identification markers for the heat-tolerant gene TT2 in rice,so as to accelerate the precise screening of heat-tolerant germplasm resources and heat-tolerant variety breeding of rice.According to the basic principle of amplification of amplification refractory mutation system PCR(ARMS-PCR)and the single nucleotide polymorphism(SNP)differences within the TT2 gene,three sets of specific primers were designed for the agarose gel electrophoresis,capillary electrophoresis,and penta-primer amplification refractory mutation system(PARMS)technology,respectively,enabling accurate discrimination of different TT2 genotypes.The agarose gel electrophoresis marker identified TT2 genotypes by the amplicon size,with high accuracy and simple operation.The capillary electrophoresis marker distinguished TT2 genotypes via the fluorescent fragment size,offering high throughput and speed.The PARMS marker achieved high-throughput and precise TT2 genotyping via a microplate reader,which was faster,simpler and more convenient.These three molecular marker systems with different detection methods can meet the detection requirements of laboratories with varied conditions,allowing the accurate identification of elite TT2 haplotypes.They provide key technical tools for pyramiding heat-tolerant genes and breeding new heat-tolerant varieties of rice,thereby promoting the development of heat-tolerant rice breeding.关键词
水稻/TT2 基因/标记开发/琼脂糖凝胶电泳/毛细管电泳/PARMS 技术Key words
rice/TT2 gene/marker development/agarose gel electrophoresis/capillary electrophoresis/PARMS technology分类
农业科技引用本文复制引用
陈若凡,陆佳雨,许学,吴爽,王沛然,张梦柯,石英尧,汪秀峰..水稻耐热基因TT2的特异性鉴定标记开发[J].杂交水稻,2026,41(2):11-20,10.基金项目
水稻种质创新与分子改良安徽省重点实验室开放基金项目(SDKF-2024-05) （SDKF-2024-05）
