浙江中医药大学学报2026，Vol.50Issue(3)：278-287,10.DOI:10.16466/j.issn1005-5509.2026.03.002
泻浊调脂方调控TLR4/MyD88/p38 MAPK信号通路改善脂代谢及炎症反应
Xiezhuo Tiaozhi Formula Improves Lipid Metabolism and Inflammatory Response by Regulating the TLR4/MyD88/p38 MAPK Signaling Pathway
摘要
Abstract
[Objective]To study the regulatory effect of Xiezhuo Tiaozhi Formula on lipid metabolism and inflammatory response in nonalcoholic fatty liver disease(NAFLD)model mice.[Methods]The NAFLD mice model was established with high-fat diet(60%energy from fat)and high-sugar drinking water(containing 55%fructose),Xiezhuo Tiaozhi Formula was administered by gavage at low,medium and high doses of 2.08,4.16 and 8.32 g·kg-1,respectively;the positive control group was administered by gavage with the contents of Polyene phosphatidylcholine capsule at 0.2 g·kg-1.The body weight,liver weight,liver lipid content and serum biochemical indexes were detected.Liver histopathology and lipid deposition were observed by HE staining and oil red O staining.The mRNA expression of sterol regulatory element-binding protein 1c(SREBP1c),cluster of differentiation 36(CD36),hormone-sensitive triglyceride lipase(HSL),carnitine palmitoyltransferase 1α(CPT1α),TNF-α,IL-6,IL-1β,NOD like receptor family pyrin domain containing 3(NLRP3),Toll-like receptor 4(TLR4)and myeloid differentiation factor 88(MyD88)mRNA were detected by Real-time qPCR,and Western blot was used to detect the protein expression of SREBP1c,CD36,TLR4,MyD88,p38 mitogen-activated protein kinase(p38 MAPK)and phosphorylated-p38(p-p38 MAPK).[Results]Compared with model group,Xiezhuo Tiaozhi Formula could effectively reduce the liver weight of NAFLD mice(P<0.01),decrease the liver lipid deposition,alleviate the liver injury,lower the blood lipid(P<0.01),inhibit the mRNA and protein expression of lipid synthesis factors SREBP1c and CD36,promote the mRNA expression of lipid decomposition factors CPT1α and HSL,reduce the mRNA expression of inflammatory factors IL-6,IL-1β,TNF-α and NLRP3,decrease the expression of TLR4 and MyD88,and reduce the phosphorylation of p38 MAPK(P<0.01).[Conclusion]Xiezhuo Tiaozhi Formula improves lipid metabolism and reduces the expression of inflammatory factors in NAFLD mice by down regulating TLR4/MyD88/p38 MAPK signaling pathway.关键词
非酒精性脂肪性肝病/多烯磷脂酰胆碱/泻浊调脂方/中药复方/脂代谢/炎症因子/TLR4/MAPKKey words
non-alcoholic fatty liver disease/polyene phosphatidylcholine/Xiezhuo Tiaozhi Formula/Compound Chinese Medicine Formulas/lipid metabolism/inflammatory factors/TLR4/MAPK分类
医药卫生引用本文复制引用
陈坤铃,窦晓兵,邱剑楠,林益游,白丹瑶,罗杨舟,周丽萍..泻浊调脂方调控TLR4/MyD88/p38 MAPK信号通路改善脂代谢及炎症反应[J].浙江中医药大学学报,2026,50(3):278-287,10.基金项目
国家自然科学基金项目(82374102、82004353) （82374102、82004353）
浙江省自然科学基金项目(LQ20H290005) （LQ20H290005）
杭州市农业与社会发展科研重点项目(202204A04) （202204A04）
浙江中医药大学科研项目(2022JKZKTS21) National Natural Science Foundation Project(82374102,82004353) （2022JKZKTS21）
Zhejiang Provincial Natural Science Foundation Project(LQ20H290005) （LQ20H290005）
Key Scientific Research Project of Agriculture and Social Development in Hangzhou City(202204A04) （202204A04）
Zhejiang Chinese Medical University Research Project(2022JKZKTS21) （2022JKZKTS21）
