中南林业科技大学学报2026，Vol.46Issue(4)：139-148,208,11.DOI:10.14067/j.cnki.1673-923x.2026.04.015
雪岭云杉响应畸形金锈菌侵染的转录组学分析
Transcriptome analysis of Picea schrenkiana to infection by Chrysomyxa deformans
摘要
Abstract
[Objective]This study conducted a transcriptomic analysis of in response to C.deformans infection in Picea schrenkiana,laying the foundation for future research into the molecular pathogenic mechanisms of C.deformans pathogenicity.[Method]Using RNA-Seq technology to compare diseased and healthy current-year tissues of P.schrenkiana,this study explored the key genes,signaling pathways,and metabolic pathways involved in the P.schrenkiana-C.deformans interaction.[Result]1)Transcriptome sequencing identifies 3 461 differentially expressed genes(DEGs),including 1 658 upregulated and 1 803 downregulated genes;2)GO analysis shows that DEGs are mainly enriched in terms like transferase activity,sugar group transfer,heme binding,tetrapyrrole binding,ADP binding,coenzyme binding,iron ion binding,UDP-glycosyltransferase activity,and oxidoreductase activity;3)KEGG analysis finds DEGs annotated in 124 KEGG pathways,with the most in metabolic pathways.DEGs are notably enriched in phenylpropanoid biosynthesis,flavonoid biosynthesis,glutathione metabolism,linoleic acid metabolism,and inositol phosphate metabolism pathways;4)Terpene synthase-related genes LOC131073238,TPS-1,8cin,TPS-Lin-2,PT5(novel.5099)and PT5(novel.5096)are downregulated,while LOC131042632 and ag5 are upregulated.Notably,DEGs LOC131042632,LOC131073238,and TPS-1,8cin are enriched in diterpenoid biosynthesis pathways;5)In the phenylpropanoid biosynthesis pathway,DEGs CYP71AU50,PER12,PER53,PER65,4CL,UGT84A2,and CCL7 are downregulated,while 36 DEGs are upregulated.[Conclusion]P.schrenkiana may respond to rust infection through strategies such as lignin synthesis,terpenoid compound production,and modulation of signaling transduction capabilities.关键词
雪岭云杉/锈病/畸形金锈菌/转录组Key words
Picea schrenkiana/rust diseases/Chrysomyxa deformans/transcriptome分类
农业科技引用本文复制引用
赛牙热木·哈力甫,刘国蓉,张思佳,李创新,刘闻慧,邓勋..雪岭云杉响应畸形金锈菌侵染的转录组学分析[J].中南林业科技大学学报,2026,46(4):139-148,208,11.基金项目
国家自然科学基金项目(31670649) （31670649）
黑龙江省自然科学基金项目(LH2023C105) （LH2023C105）
黑龙江省重点研发项目(2023ZX02B05) （2023ZX02B05）
石河子大学高层次人才科研启动项目(RCZK2021B23) （RCZK2021B23）
石河子大学自主资助支持专项(ZZZC2022009). （ZZZC2022009）
