中南民族大学学报（自然科学版）2026，Vol.45Issue(4)：483-488,6.DOI:10.20056/j.cnki.ZNMDZK.20260707
L6细胞中稳定敲除AMPKα1对抗糖尿病药物发挥作用的影响
Effect of stable knockout of AMPKα1 on anti-diabetic medication function in L6 cells
摘要
Abstract
The consumption of glucose by muscle cells is a key step in determining the level of blood sugar in the body.Adenosine 5'-monophosphate-activated protein kinase(AMPK),a key pathway in diabetes adenosine monophosphate,promotes glucose uptake in muscle cells when activated by phosphorylation.In order to explore the key role of AMPKαsubunit in the treatment of diabetes,AMPKα1 protein in L6 rat skeletal muscle cells was knocked out by using regularly clustered interspaced short palindromic repeats(CRISPR/Cas9)to explore the effect of AMPKα1 subunit deletion in L6 cells on the treatment of diabetes.The results showed that the metformin-promoted phosphorylation of AMPKα subunit completely disappeared when AMPKα was deleted,and the effect of metformin on glucose uptake in normal cells showed a decreasing trend in knockout cells.It reveals the key position of AMPKα1 subunit in the action of diabetic drugs,and provides an important experimental model for precise targeted drug therapy and drug mechanism exploration.关键词
单磷酸腺苷活化蛋白激酶/葡萄糖摄取/规律成簇的间隔短回文重复系统(CRISPR/Cas9)/单磷酸腺苷活化蛋白激酶α1亚基Key words
AMPK/glucose uptake/CRISPR/Cas9/AMPKα1分类
生物科学引用本文复制引用
赵平,田贵林,杨新洲..L6细胞中稳定敲除AMPKα1对抗糖尿病药物发挥作用的影响[J].中南民族大学学报（自然科学版）,2026,45(4):483-488,6.基金项目
国家自然科学基金资助项目(82474138) （82474138）
湖北省自然科学基金资助项目(2023AFC011) （2023AFC011）
中央高校基本科研业务费专项资金资助项目(CZY23021) （CZY23021）
