中南民族大学学报（自然科学版）2026，Vol.45Issue(4)：496-504,9.DOI:10.20056/j.cnki.ZNMDZK.20260731
基于转录组测序解析白花前胡早薹早花相关基因的动态变化
Dynamic changes analysis of genes related to early bolting and flowering in Peucedanum praeruptorum based on transcriptome sequencing
摘要
Abstract
The traditional Chinese medicine Peucedanum praeruptorum is usually used in medicine with its roots.The early bolting and early flowering often occurs in artificial planting and leads to rapid lignification of roots,the active ingredient content decreased sharply.However,the cause and mechanism of this appearance have not been clarified.Transcriptome sequencing was completed using experimental samples from three different growth stages:non-bolting,bolting and flowering.A total of 83505 transcripts were identified,of which 12623 genes were significantly different.Further enrichment of distinct genes showed that the pathways closely related to early flowering include photosynthesis-antenna protein,plant hormone signal transduction and circadian rhythm pathway.Through comparative database analysis,91 differential expressed genes(DEGs)were identified.Based on the significance of expression level variations,12 candidate genes(SOC1-1,SOC1-2,DFC,FT,VRN1,PHYA,COL9,FLD,FCA,RGL2,EMF2,EFL4)were subsequently selected for qRT-PCR validation.The verification results demonstrated a high degree of consistency with transcriptome sequencing data,suggesting these 12 genes may be associated with early bolting and flowering mechanisms.关键词
白花前胡/转录组测序/早薹早花/转录调控Key words
Peucedanum praeruptorum/RNA-seq/early bolting and flowering/transcriptional regulation分类
生物科学引用本文复制引用
蒲玥,邹家辉,张宗铭,徐俊伟,王春台,谭艳平..基于转录组测序解析白花前胡早薹早花相关基因的动态变化[J].中南民族大学学报（自然科学版）,2026,45(4):496-504,9.基金项目
中央高校基本科研业务费专项资金资助项目(CZY23001) （CZY23001）
