作物学报2026，Vol.52Issue(5)：1291-1308,18.DOI:10.3724/SP.J.1006.2026.53078
玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应
Genome-wide identification of the maize MAPK gene family and its response to Fusarium verticillioides infection
摘要
Abstract
Fusarium ear rot(FER),caused by Fusarium verticillioides,is one of the most destructive fungal diseases limiting maize(Zea mays L.)production.The mitogen-activated protein kinase(MAPK)cascade is a central regulator of plant growth,development,and stress responses.Previous studies have shown that ZmMAPKs can enhance resistance to fungal pathogens by upregulating defense-related genes and activating key enzymes;however,the roles of individual MAPK family members in path-ways associated with FER resistance remain unclear.Here,we conducted a comprehensive analysis of the ZmMAPK gene family,including genome-wide identification and characterization,chromosomal localization,phylogenetic reconstruction,collinearity analysis,promoter cis-element prediction,tissue-specific expression profiling,and expression responses to F.verticillioides infec-tion.In total,24 MAPK genes were identified in the maize B73 reference genome(V5).Phylogenetic analysis grouped these genes into four subgroups,with highly conserved exon-intron structures within each subgroup.Groups A,B,and C contain the canonical Thr-Glu-Tyr(TEY)activation motif,whereas group D contains the Thr-Asp-Tyr(TDY)motif.Expression analyses showed pronounced tissue-specific differences,suggesting functional diversification within the family.RT-qPCR further indicated that,after F.verticillioides inoculation,ZmMAPK12,ZmMAPK22,ZmMAPK23,and ZmMAPK24 were differentially expressed between the resistant inbred line Qi 319 and the susceptible inbred line B73.Sequence comparisons revealed that,in addition to SNPs,structural variants such as transposon insertions occurred in both promoter and coding regions of these genes.Together,these results suggest that these MAPKs may participate in regulatory pathways controlling maize responses to F.verticillioides infection.This study provides a foundation for elucidating how MAPK cascades contribute to FER resistance and offers candidate genes to support genetic improvement of disease-resistant maize varieties.关键词
玉米/MAPK基因家族/穗腐病/拟轮枝镰孢菌/表达模式Key words
maize/MAPK gene family/Fusarium ear rot/Fusarium verticillioides/expression pattern引用本文复制引用
孙淑凤,许振南,黄嘉鑫,翁建峰,李新海..玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J].作物学报,2026,52(5):1291-1308,18.基金项目
本研究由国家重点研发计划项目(2023YFD1201102)资助.This study was supported by the National Key Research&Development Program of China(2023YFD1201102). （2023YFD1201102）
