花生WOX基因家族的全基因组分析及不定芽再生候选基因的鉴定
Genome-wide analysis of the WOX gene family and identification of candidate genes for adventitious shoot regeneration in peanut
摘要
Abstract
The WUSCHEL-related homeobox(WOX)transcription factor family plays central roles in maintaining stem cell ho-meostasis and regulating organogenesis in plants.Genetic transformation of peanut(Arachis hypogaea L.),a globally important oilseed crop,remains severely limited by low regeneration efficiency.Here,we conducted a genome-wide identification and characterization of WOX genes in the peanut cultivar'Fuhuasheng'to pinpoint candidates potentially involved in organ regenera-tion and to provide a basis for overcoming regeneration-related constraints in peanut transformation.We identified 20 WOX genes and systematically analyzed their phylogenetic relationships,conserved domains,collinearity,and promoter features,and then profiled their expression across tissues and during adventitious shoot regeneration using transcriptome data.Phylogenetic analysis clustered the AhWOX genes into three clades(ancient,intermediate,and WUS).All AhWOX proteins contained a conserved ho-meodomain(HD),and several WUS-clade members also carried the WUS-box and EAR motifs.Collinearity analysis indicated that AhWOX7,AhWOX12,and AhWOX14 are orthologs of Arabidopsis thaliana WUS and WOX5 and of the soybean regenera-tion-related gene GmWOX18,respectively.However,these orthologs showed only low expression,whereas the ancient-clade genes AhWOX13 and AhWOX20 were consistently highly expressed across all stages of adventitious shoot regeneration.Promoter analysis revealed enrichment of cis-elements associated with meristem activity,and phylogenetic evidence suggested that Ah-WOX13 and AhWOX20 are orthologous to AtWOX14,a reported positive regulator of shoot regeneration.Collectively,our results highlight AhWOX13 and AhWOX20 as promising candidates for improving regeneration capacity in peanut,providing a frame-work to enhance peanut transformation and accelerate functional genomics and trait engineering in oilseed crops.关键词
花生/WOX转录因子/全基因组分析/器官再生/AhWOX13/AhWOX20Key words
Arachis hypogaea L./WOX transcription factors/whole-genome analyses/organ regeneration/AhWOX13/AhWOX20引用本文复制引用
谷春苗,陈小平,谭斌,余倩霞,王润风,黄璐,刘浩,鲁清,李海芬,李少雄,何双呈,洪彦彬..花生WOX基因家族的全基因组分析及不定芽再生候选基因的鉴定[J].作物学报,2026,52(5):1326-1340,15.基金项目
本研究由广东省农业科学院协同创新中心项目(XTXM202203),国家自然科学基金项目(32401893),广东省基础与应用基础研究基金项目(2024A1515010511),广东省农业科学院作物研究所所长基金/广东省农作物遗传改良重点实验室开放基金项目(202505,202604),广东省农业科学院科技人才引进专项资金项目(R2024YJ-YB1002)和广东省农业科学院现代种业创新能力提升工程项目资助.This study was supported by the Project of Collaborative Innovation Center of GDAAS(XTXM202203),the National Natural Science Foundation of China(32401893),the Guangdong Basic and Applied Basic Research Foundation(2024A1515010511),the Foundation of Director of Crop Research Institute of Guangdong Academy of Agriculture Sciences/Open Fund of Guangdong Provincial Key Laboratory of Crop Genetic Improvement(202505,202604),the Special Fund Project for the Introduction of Scientific and Technological Talents of the Guangdong Academy of Agricultural Sciences(R2024YJ-YB1002),and the Modern Seed Industry Innovation Capability Enhancement Pro-ject of Guangdong Academy of Agricultural Sciences. （XTXM202203）
