作物学报2026，Vol.52Issue(5)：1388-1400,13.DOI:10.3724/SP.J.1006.2026.54134
EMS诱导糜子优异性状突变体的筛选及表型分析
Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet
摘要
Abstract
Ethyl methanesulfonate(EMS)mutagenesis is an effective approach for creating genetic diversity in broomcorn millet(Panicum miliaceum L.)and for developing novel mutant lines with improved agronomic traits.Using the landrace'Heishuzi'as the starting material,we conducted a two-factor completely crossed experimental design with six EMS concentrations(0,0.4%,0.6%,0.8%,1.0%,and 1.2%)and five soaking durations(6,8,10,12,and 14 h),yielding 30 treatment combinations.EMS sig-nificantly inhibited seed germination,and germination rate decreased progressively with increasing EMS concentration and longer soaking duration.Based on phenotypic variation,18 distinct mutant phenotypes were identified,including dwarf,compact panicle,leaf-color variants,early maturity,and single tillering.In the M2 population,297 mutant plants were recovered(mutation frequency,28.89%);dwarf mutants were the most common(35 plants),whereas single-tillering mutants were the least frequent(5 plants).In the M3 population,215 mutant plants were obtained(mutation frequency,20.91%),with early-maturing mutants being the most abundant(35 plants)and single-tillering mutants remaining relatively rare(6 plants).In addition,215 M3 lines were genotyped with 85 broomcorn millet-specific SSR markers;12 markers with clear amplification and high polymorphism were selected,and 44 mutant plants were identified(variation frequency,4.3%).Electrophoretic banding patterns indicated 32 mutants with additional bands,6 with missing bands,and 6 with locus variation.Agronomic evaluation of these mutants identified 1 com-pact-panicle plant,21 dwarf plants(60-70 cm in height),11 early-maturing(55-60 days)plants,and 2 single-tillering plants.Collectively,these results provide valuable germplasm resources for genetic improvement and breeding in broomcorn millet.关键词
糜子/EMS/SSR/农艺性状/突变体Key words
broomcorn millet/EMS/SSR/agronomic traits/mutant plants引用本文复制引用
张颖星,Bheel Chander Kumar,宋裕祯,王跃,曹越,Khound Rituraj,Santra Dipak Kumar,曹晓宁,王瑞云..EMS诱导糜子优异性状突变体的筛选及表型分析[J].作物学报,2026,52(5):1388-1400,13.基金项目
本研究由财政部和农业农村部现代农业产业技术体系建设专项(CARS-06-14.5-A16),山西省现代农业产业技术体系建设(杂粮)专项资金项目(2026CYJSTX03-12)和山西省重点研发计划项目(2022ZDYF110)资助.This study was supported by the China Agriculture Research System of MOF and MARA(CARS-06-14.5-A16),the Special Fund for Con-struction of Modern Agricultural Industrial Technology System of Shanxi Province(2026CYJSTX03-12),and the Key Research&Develop-ment Project of Shanxi Province(2022ZDYF110). （CARS-06-14.5-A16）
