作物学报2026，Vol.52Issue(5)：1430-1441,12.DOI:10.3724/SP.J.1006.2026.54123
木薯分枝发育过程的动态转录组分析与关键基因发掘
Dynamic transcriptome analysis and key gene discovery during cassava branching development
摘要
Abstract
Branching is a key trait that determines plant architecture and yield in cassava.However,its molecular regulatory mechanisms remain unclear.In this study,we used the multi-branching cassava cultivar SC5 as material.We systematically analyzed the regulatory network of cassava branching by measuring phytohormone levels at different developmental stages and integrating transcriptome sequencing analysis.The results showed that during cassava branching,the contents of auxin and cytokinin continuously decreased.Gibberellin content initially increased and reached a peak value at the stage of axil-lary bud activation,and then decreased.Correspondingly,the expression of genes involved in auxin biosynthesis and sig-naling transduction were consistently down-regulated.Meanwhile,the gibberellin-related genes were significantly up-regulated.Furthermore,seven key transcription factors were identified,including MeTCP(2),MeMADS(2),MeAP2(1),MeHD-ZIP(1),and MeERF(1).Their expression patterns were closely associated with branching development.In conclu-sion,branching development in cassava was coordinately regulated by the dynamic balance of phytohormones and a multi layered transcriptional regulatory network.关键词
木薯/分枝/转录组/植物激素/转录因子Key words
cassava/branching/transcriptome/plant hormone/transcription factor引用本文复制引用
蔡兆琴,何观咏,何文,阮丽霞,梁振华,李永珍,李恒锐,陈会鲜..木薯分枝发育过程的动态转录组分析与关键基因发掘[J].作物学报,2026,52(5):1430-1441,12.基金项目
本研究由国家重点研发计划项目(2023YFD1600600),广西青年科学基金项目(2024GXNSFBA010382,2024GXNSFAA010006),广西农业科学院稳定资助科研团队项目(桂农科 2021YT157)和广西南亚热带农业科学研究所自筹项目(NYS2025ZC03)资助.This study was supported by the National Key Research and Development Program of China(2023YFD1600600),the Guangxi Youth Science Foundation Project(2024GXNSFBA010382,2024GXNSFAA010006),the Guangxi Academy of Agricultural Sciences Stable Funding Re-search Team Project(Guinongke 2021YT157),and the Self-funded Project of Guangxi South Subtropical Agricultural Science Research Institute(NYS2025ZC03). （2023YFD1600600）
