植物遗传资源学报2026，Vol.27Issue(4)：675-689,15.DOI:10.13430/j.cnki.jpgr.20251212001
小麦地方品种核心种质赤霉病抗性鉴定及抗性基因检测
Identification of Fusarium Head Blight Resistance and Detection of Resistance Genes in a Core Collection of Wheat Landraces
摘要
Abstract
Fusarium head blight(FHB)is one of the most devastating diseases affecting wheat production.Breeding disease-resistant varieties is the most economical and effective method of controlling FHB.Wheat landraces have been identified as a valuable source of germplasm for FHB resistance.In this study,we evaluated the FHB resistance of 311 core collection of wheat landraces from China's southern winter wheat region,combining the results with the previously reported major FHB resistance genes/QTL(Fhb1,Fhb2,Fhb4,Fhb5,Fhb8,Fhb9,and QFhb.yas-2DL).This allowed us to assess the effects of different resistance genes and their combinations on resistance,and to screen out excellent new sources of resistance without known genes.Among the 311 landraces,158 consistently exhibited moderate resistance or higher(50.80％)over three years,of which 65 demonstrated resistance in all three years(20.90％).The molecular marker detection results indicated that the following detection frequencies of the known resistance genes/QTL,from highest to lowest were QFhb.yas-2DL(89.39％),Fhb5(55.95％),Fhb1(47.59％),Fhb2(27.65％),Fhb4(23.15％)and Fhb8(6.75％).No varieties carrying Fhb9 were detected.Of the seven reported FHB resistance genes/QTL,varieties carrying Fhb1 exhibited the lowest average percentage of symptomatic spikelets(PSS:21.47％)and had the most significant impact on reducing PSS.The pyramiding of multiple resistance genes has the potential to enhance FHB resistance.An analysis of resistance gene combinations revealed that,among the two-gene combinations,the FHB resistance exhibited by the Fhb1+QFhb.yas-2DL combination was superior to that of the Fhb5+QFhb.yas-2DL combination.Among the three-gene combinations,the Fhb1+Fhb2+QFhb.yas-2DL combination performed better.In the four-gene combinations,the FHB resistance performance of Fhb1+Fhb4+Fhb5+QFhb.yas-2DL and Fhb1+Fhb2+Fhb5+QFhb.yas-2DL were comparable.Additionally,this study identified three promising new resistance sources of resistance:Chushanbao,Youmangyuqiumai and Caomai.These varieties did not carry any known resistance genes/QTL,exhibited moderate resistance over three consecutive years.Consequently,these varieties have the potential to be utilized in the future for the identification of novel FHB resistance genes or QTLs.关键词
小麦/赤霉病/地方品种/分子标记/抗性基因Key words
wheat/Fusarium head blight/landraces/molecular markers/resistance gene引用本文复制引用
陈雨心,吴纪中,刘颖,翟文玲,刘彩云,蔡瑾,刘富杰,付必胜,林芝欣,许峰..小麦地方品种核心种质赤霉病抗性鉴定及抗性基因检测[J].植物遗传资源学报,2026,27(4):675-689,15.基金项目
生物育种钟山实验室(ZSBBL-KY2023-02-1) （ZSBBL-KY2023-02-1）
国家自然科学基金(32101792) （32101792）
江苏省自然科学基金(BK20241166) （BK20241166）
江苏省种业振兴揭榜挂帅项目(JBGS[2021]013) （JBGS[2021]013）
农业农村部种业管理司小麦种质资源精准鉴定任务Zhongshan Biological Breeding Laboratory(ZSBBL-KY2023-02-1) （ZSBBL-KY2023-02-1）
National Natural Science Foundation of China(32101792) （32101792）
Natural Science Foundation of Jiangsu Province(BK20241166) （BK20241166）
Jiangsu Seed Industry Revitalization Project(JBGS[2021]013) （JBGS[2021]013）
Precision Identification Task for Wheat Germplasm Resources,Department of Seed Industry Administration,Ministry of Agriculture and Rural Affairs （）
