植物遗传资源学报2026，Vol.27Issue(4)：763-775,13.DOI:10.13430/j.cnki.jpgr.20251125003
苦荞FtADPG2蛋白在立枯丝核菌RsEgD毒性蛋白致病过程中的作用机制研究
Mechanism of FtADPG2 Protein in Tartary Buckwheat During the Pathogenesis of Rhizoctonia solani RsEgD Virulence Factors
摘要
Abstract
Sheath blight caused by Rhizoctonia solani poses a serious threat to the production of tartary buckwheat(Fagopyrum tataricum).Previous work by our group suggested that FtADPG2,a gene from the polygalacturonase(PG)family,might be associated with sheath blight resistance in tartary buckwheat.To gain deeper insights into the functional role of this gene in disease resistance,we cloned FtADPG2 from the cultivar Zhongku 3.The gene encodes a stable protein with a molecular weight of 41.65 kDa.Expression analysis revealed that FtADPG2 transcription was significantly induced upon R.solani infection,and this induction showed a coordinated upregulation with the expression of the pathogenesis-related protein PR1 and the accumulation of jasmonic acid.Heterologous overexpression of FtADPG2 significantly enhanced disease susceptibility in both tobacco and Arabidopsis.Haplotype analysis identified a natural variation(Ala22Val)within the first exon,defining a haplotype designated Hap2.Notably,Hap2 was associated with higher enzymatic activity and conferred a greater susceptibility phenotype.Based on transcriptomic data and AlphaFold structure prediction,we identified a candidate fungal virulence protein,RsEgD.Protein-protein interaction between FtADPG2 and RsEgD was subsequently confirmed by yeast two-hybrid and luciferase complementation imaging assays.Our results demonstrate that FtADPG2 is a key susceptibility gene in tartary buckwheat,promoting infection through a physical interaction with the pathogen effector RsEgD.This study provides crucial targets and a theoretical foundation for elucidating the molecular mechanisms of F.tataricum-R.solani interaction and for breeding buckwheat varieties with enhanced disease resistance.关键词
苦荞/立枯丝核菌/FtADPG2/感病基因/茉莉酸/病程相关蛋白Key words
tartary buckwheat/Rhizoctonia solani/FtADPG2/susceptibility gene/jasmonic acid/pathogenesis-related protein引用本文复制引用
范红萍,樊冬晴,朱剑锋,王韵雪,王俊珍,李周,黄旭,方正武,周美亮..苦荞FtADPG2蛋白在立枯丝核菌RsEgD毒性蛋白致病过程中的作用机制研究[J].植物遗传资源学报,2026,27(4):763-775,13.基金项目
国家自然科学基金(32161143005) （32161143005）
云南省科技人才与平台计划(202505AF350051) （202505AF350051）
昭通市院士专家工作站项目(2024ZTYX06) （2024ZTYX06）
凉山州农业科技"揭榜挂帅"项目(LSNY2024003)National Natural Science Foundation of China(32161143005) （LSNY2024003）
Yunnan Provincial Science and Technology Talent and Platform Program(202505AF350051) （202505AF350051）
Zhaotong City Academician and Expert Workstation Project(2024ZTYX06) （2024ZTYX06）
Liangshan Prefecture Agricultural Science and Technology"Unveiling and Assuming Leadership"Project(LSNY2024003) （LSNY2024003）
